Analysis of Isoenzyme Patterns of Acid Phosphatase in Acute Leukemias

Overview

Journal Blut

Specialty Hematology

Date 1983 Aug 1

PMID 6575840

Citations 2

Authors

H G Drexler

G Gaedicke

Affiliations

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Abstract

The status of acid phosphatase isoenzymes was evaluated in cells of patients with acute lymphocytic leukemias or lymphomas by analytical isoelectric focusing in polyacrylamide gels (IEF) on horizontal thin-layer slabs. The isoenzyme patterns were correlated with routine immunological cell surface markers and the relationship of enzyme activity to specific immunological subclasses of ALL is discussed. By isoelectric focusing up to five isoenzyme groups (I-V) containing several isoenzyme were observed. No leukemia specific or additional isoenzyme could be demonstrated. This biochemical characterization showed a marked heterogeneity within two major immunologic subgroups indicating that various differentiation stages of cell maturation could be involved in cALL and T-ALL. According to their degree of maturation along T-cell differentiation axis the leukemic cells displayed no enzyme activity, weak isoenzyme bands or the incomplete or complete isoenzyme pattern seen with normal lymphocytes from human tonsils which were used as controls. The investigation of specific enzymatic patterns can lead to a further definition of subsets of acute leukemias and give insight into lymphopoietic differentiation.

Citing Articles

Leukemic cell differentiation in childhood leukemias. Analysis by enzyme markers.

Gaedicke G, Drexler H Eur J Pediatr. 1984; 142(3):157-64.

PMID: 6236088 DOI: 10.1007/BF00442441.

Biochemical enzyme analysis in acute leukaemia.

Drexler H, Gaedicke G, Minowada J J Clin Pathol. 1985; 38(2):117-27.

PMID: 2981904 PMC: 499089. DOI: 10.1136/jcp.38.2.117.

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