Human Natural Killing Against Ovarian Carcinoma

Overview

Journal Br J Cancer

Specialty Oncology

Date 1983 May 1

PMID 6573908

Citations 4

Authors

H Shau

H S Koren

J R Dawson

Affiliations

Soon will be listed here.

Abstract

Natural killing (NK) by lymphocytes from normal individuals against primary and established ovarian carcinoma cell lines was tested in short-term chromium release assays. Two established cell lines and 5/6 primary cell lines tested showed significant susceptibility to NK. Primary cell lines are, in general, less sensitive to NK than long-term cultured target cells. A common NK recognition determinant on ovarian carcinoma cells and on the erythroleukaemic K562 cells was demonstrated by cold target inhibition assays. The recognition structure was also present on an ovarian cell line resistant to NK but not on the insensitive leukaemic cell line, SB. The activity against ovarian carcinoma cells was associated with the presence of large granular lymphocytes (LGL) previously shown to be the major effector cells against K562 targets. In fractions obtained by Percoll gradient centrifugation of lymphocytes, only fractions with a high content of LGL demonstrated good NK activity. LGL mediated NK against both non-adherent K562 and the adherent ovarian carcinoma target cells independent of monocytes.

Citing Articles

The role of the lysosome in natural killing: inhibition by lysosomotropic vital dyes.

Shau H, Dawson J Immunology. 1984; 53(4):745-51.

PMID: 6500627 PMC: 1454886.

Human ovarian carcinoma: evidence for patient-related differences in susceptibility to cytotoxic effectors that attack different cellular subpopulations within a tumour.

Clark D, Hirte H, Buick R Br J Cancer. 1988; 58(4):415-8.

PMID: 3264715 PMC: 2246786. DOI: 10.1038/bjc.1988.232.

Studies on cytotoxicity generated in human mixed lymphocyte culture. IV. Interleukin 2 alone or from mixed lymphocyte culture yields natural killer-like cytotoxic cells distinct from allospecific cytotoxic T lymphocytes.

Shau H, GRAY J, Golub S Cancer Immunol Immunother. 1988; 27(3):255-60.

PMID: 2972366 PMC: 11038593. DOI: 10.1007/BF00205448.

A preliminary study on natural killer activity in patients with gynecologic malignancies.

Ma D, Gu M, Liu B J Tongji Med Univ. 1990; 10(3):159-63.

PMID: 1701494 DOI: 10.1007/BF02986455.

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