Quantitative Ultrastructural Studies on Rat Gastric Zymogen Cells Under Different Physiological and Experimental Conditions

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1978 May 29

PMID 657245

Citations 4

Authors

H F Helander

Affiliations

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Abstract

Quantitative electron microscopical measurements performed on gastric zymogen cells were aimed at determinations of size and volume density of the zymogen granules, and size of cell and nuclear profiles. 17 groups of rats each comprising 6-8 animals were investigated: five of these groups were used to study the influence of fasting and feeding, three groups were killed at different intervals after a pilocarpine injection, and four other groups were investigated after an atropine injection. The remaining five groups of rats were operated on: vagotomy was performed on one group, vagotomy + pyloroplasty on another, pyloroplasty on the third group, and antrectomy on the last two groups. The operated rats were sacrificed 4 or 10 weeks after the operations. Pilocarpine was more effective than feeding in reducing the size and the amount of zymogen granules. After atropine the size and amount of zymogen granules tended to increase. Ten weeks after pyloroplasty, vagotomy + pyloroplasty, or antrectomy the mean size of the zymogen cells was reduced. Loss of trophic vagal impulses, duodenal regurgitation, and abnormal serum gastrin levels are factors which might be responsible for the zymogen cell hypotrophy in operated rats.

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References

REHM W . Some aspects of the problem of gastric hydrochloric acid secretion. Arch Intern Med. 1972; 129(2):270-8. View

Bolender R . Stereological analysis of the guinea pig pancreas. I. Analytical model and quantitative description of nonstimulated pancreatic exocrine cells. J Cell Biol. 1974; 61(2):269-87. PMC: 2109295. DOI: 10.1083/jcb.61.2.269. View

Martin F, MACLEOD I, Sircus W . Effects of antrectomy on the fundic mucosa of the rat. Gastroenterology. 1970; 59(3):437-44. View

Willems G, Galand P, Vansteenkiste Y, Zeitoun P . Cell population kinetics of zymogen and parietal cells in the stomach of mice. Z Zellforsch Mikrosk Anat. 1972; 134(4):505-18. DOI: 10.1007/BF00307670. View

Amsterdam A, JAMIESON J . Studies on dispersed pancreatic exocrine cells. I. Dissociation technique and morphologic characteristics of separated cells. J Cell Biol. 1974; 63(3):1037-56. PMC: 2109378. DOI: 10.1083/jcb.63.3.1037. View

Lundell L, SVENSSON S . Alterations in secretory patterns following vagotomy in rats with Pavlov or Heidenhain pouches. J Physiol. 1973; 234(3):703-21. PMC: 1350695. DOI: 10.1113/jphysiol.1973.sp010368. View

CREAN G, Gunn A, Rumsey R . The effects of vagotomy on the gastric mucosa of the rat. Scand J Gastroenterol. 1969; 4(8):675-80. DOI: 10.3109/00365526909180653. View

Langley J . On the Histology of the Mammalian Gastric Glands and the Relation of Pepsin to the Granules of the Chief-Cells. J Physiol. 1882; 3(3-4):269-91. PMC: 1484932. DOI: 10.1113/jphysiol.1882.sp000100. View

Nevalainen T . Effects of pilocarpine stimulation on rat pancreatic acinar cells. An electron microscopic study with morphometric analysis. Acta Pathol Microbiol Scand Suppl. 1970; 210:Suppl 210:1+. View

10.

Weber J . The basophilic substance of the gastric chief cells and its relation to the process of secretion. Acta Anat Suppl (Basel). 1958; 33(1):1-78. View

11.

Helander H . Stereological changes in rat parietal cells after vagotomy and antrectomy. Gastroenterology. 1976; 71(6):1010-8. View

12.

Wahlin T, BLOOM G, Carlsoo B, Rhodin L . Effects of fasting and refeeding on secretory granules of the mouse gallbladder epithelium. A quantitative electron microscopic study. Gastroenterology. 1976; 70(3):353-8. View

13.

Weibel E, Staubli W, Gnagi H, Hess F . Correlated morphometric and biochemical studies on the liver cell. I. Morphometric model, stereologic methods, and normal morphometric data for rat liver. J Cell Biol. 1969; 42(1):68-91. PMC: 2107575. DOI: 10.1083/jcb.42.1.68. View

14.

JAMIESON J, Palade G . Synthesis, intracellular transport, and discharge of secretory proteins in stimulated pancreatic exocrine cells. J Cell Biol. 1971; 50(1):135-58. PMC: 2108418. DOI: 10.1083/jcb.50.1.135. View

15.

Cope G, Williams M . Improved preservation of parotid tissue for electron microscopy. A method permitting the collection of valid stereological data. J Cell Biol. 1974; 60(1):292-7. PMC: 2109147. DOI: 10.1083/jcb.60.1.292. View

16.

Helander H . Ultrastructure of fundus glands of the mouse gastric mucosa. An electron microscopical study in fasted and refed animals with observations on ultrastructure after different fixation and embedding procedures. J Ultrastruct Res. 1962; Suppl 4:1-123. DOI: 10.1016/s0022--532(0()62)80047--1. View

17.

Johnson L, Lichtenberger L, Copeland E, Dudrick S, Castro G . Action of gastrin on gastrointestinal structure and function. Gastroenterology. 1975; 68(5 Pt 1):1184-92. View

18.

Carlsoo B, Danielsson A, Helander H . Effects of starvation on amylase storage in mouse pancreas and parotid gland. A biochemical and morphometric study. Acta Hepatogastroenterol (Stuttg). 1974; 21(1):48-57. View

19.

Amsterdam A, Ohad I, Schramm M . Dynamic changes in the ultrastructure of the acinar cell of the rat parotid gland during the secretory cycle. J Cell Biol. 1969; 41(3):753-73. PMC: 2107813. DOI: 10.1083/jcb.41.3.753. View

20.

Ichikawa A . FINE STRUCTURAL CHANGES IN RESPONSE TO HORMONAL STIMULATION OF THE PERFUSED CANINE PANCREAS. J Cell Biol. 1965; 24:369-85. PMC: 2106586. DOI: 10.1083/jcb.24.3.369. View