[3H]Pirenzepine Identifies Putative M1 Muscarinic Receptors in Human Stellate Ganglia

Overview

Journal Brain Res

Specialty Neurology

Date 1984 Jan 2

PMID 6546354

Citations 8

Authors

M Watson

W R Roeske

P C Johnson

H I Yamamura

Affiliations

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Abstract

The specific binding of [3H]pirenzepine ( [3H]PZ) and [3H](-) quinuclidinyl benzilate ( [3H](-)QNB) was investigated in homogenates of human stellate ganglia. [3H]PZ saturation isotherms yielded a Kd of 14 nM and Bmax of 16.7 fmol/mg protein, while [3H](-)QNB binding curves yielded a Kd of 59 pM and Bmax of 33.0 fmol/mg protein. This represents the greatest proportion of high affinity [3H]PZ labeling, relative to [3H](-)QNB, seen in any peripheral tissue examined thus far.

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