Immunological and Enzymatic Studies of Erythrocytic Delta-aminolevulinate Dehydratase. Comparison of Results Obtained in Normal and Lead-exposed Subjects

Overview

Journal Int Arch Occup Environ Health

Specialty Environmental Health

Date 1984 Jan 1

PMID 6526504

Citations 4

Authors

C Boudene

E Comoy

C Bohuon

Affiliations

Soon will be listed here.

Abstract

The delta-aminolevulinate dehydratase (ALA.D) quantitative assay on a centrifugal fast analyser showed that subjects whose blood-lead level varies between 30 and 75 micrograms/100 ml (1.5 to 3.75 microM/l) react to blood intoxication by synthesizing de novo an amount of enzyme correlating to blood-lead levels. At higher concentrations, the reactional synthesis occurs very rarely. These results suggest that enzyme is constitutive, but also inductible as soon as its substrate accumulates; this last ability may disappear at high blood-lead levels: a hypothesis is proposed thereafter.

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References

Tomokuni K . Zeta-aminolevulinic acid dehydratase test for lead exposure. Arch Environ Health. 1974; 29(5):274-81. DOI: 10.1080/00039896.1974.10666587. View

MEREDITH P, Moore M, GOLDBERG A . Effects of aluminium, lead and zinc on delta-aminolaevulinic acid dehydratase. Enzyme. 1977; 22(1):22-7. DOI: 10.1159/000458503. View

Chiba M . Activity of erythrocyte delta-aminolevulinic acid dehydrase and its change by heat treatment as indices of lead exposure. Br J Ind Med. 1976; 33(1):36-42. PMC: 1008100. DOI: 10.1136/oem.33.1.36. View

Berlin A, Schaller K . European standardized method for the determination of delta-aminolevulinic acid dehydratase activity in blood. Z Klin Chem Klin Biochem. 1974; 12(8):389-90. View

Candura F, Franco G, POZZI U . [Influence of the heat on erythrocyte ALA-dehydratase activity in the rabbit and man. I. Findings in lead poisoning]. Lav Um. 1971; 23(1):1-10. View

Morgan J, Burch H . Erythrocyte delta-aminolevulinic acid dehydratase activity as a measure of lead exposure. J Lab Clin Med. 1975; 85(5):746-55. View

Tsukamoto I, Yoshinaga T, Sano S . Zinc and cysteine residues in the active site of bovine liver delta-aminolevulinic acid dehydratase. Int J Biochem. 1980; 12(5-6):751-6. DOI: 10.1016/0020-711x(80)90157-3. View

HERNBERG S, Nikkanen J, Mellin G, Lilius H . Delta-aminolevulinic acid dehydrase as a measure of lead exposure. Arch Environ Health. 1970; 21(2):140-5. DOI: 10.1080/00039896.1970.10667211. View

Farkas W . Effect of plumbous ion on messenger RNA. Chem Biol Interact. 1975; 11(4):253-63. DOI: 10.1016/0009-2797(75)90079-4. View

10.

Roels H, Buchet J, Lauwerys R . Inhibition of human erythrocyte delta-aminolevulinate dehydratase by lead. In vitro artifact or real phenomenon in vivo?. Int Arch Arbeitsmed. 1974; 33(4):277-84. DOI: 10.1007/BF00538932. View

11.

DOWDLE E, Wilson E, Burger P . Symptomatic porphyria I. The effects of lead on the synthesis of porphyrin and haem. S Afr Med J. 1971; :38-45. View

12.

Nakao K, Wada O, Yano Y . Delta-aminolevulinic acid dehydratase activity in erythrocytes for the evaluation of lead poisoning. Clin Chim Acta. 1968; 19(2):319-25. DOI: 10.1016/0009-8981(68)90341-0. View

13.

Sakai T, Yanagihara S, Ushio K . Restoration of lead-inhibited 5-aminolevulinate dehydratase activity in whole blood by heat, zinc ion, and (or) dithiothreitol. Clin Chem. 1980; 26(5):625-8. View

14.

Schlegel H, Kufner G . Long-term observation of biochemical effects of lead in human experiments. J Clin Chem Clin Biochem. 1979; 17(4):225-33. DOI: 10.1515/cclm.1979.17.4.225. View

15.

HAPKE H, Prigge E . Interactions of lead and glutathione with delta-aminolevulinic acid dehydratase. Arch Toxikol. 1973; 31(2):153-61. DOI: 10.1007/BF00310393. View

16.

Border E, Cantrell A, KILROE-SMITH T . The in vitro effect of zinc on the inhibition of human delta-aminolevulinic acid dehydratase by lead. Br J Ind Med. 1976; 33(2):85-7. PMC: 1008109. DOI: 10.1136/oem.33.2.85. View

17.

Raghavan S, Gonick H . Isolation of low-molecular-weight lead-binding protein from human erythrocytes. Proc Soc Exp Biol Med. 1977; 155(2):164-7. DOI: 10.3181/00379727-155-39766. View

18.

Werner C, Krebs B, Keith G, DIRHEIMER G . Specific cleavages of pure tRNAs by plumbous ions. Biochim Biophys Acta. 1976; 432(2):161-75. DOI: 10.1016/0005-2787(76)90158-1. View

19.

Fujita H, Orii Y, Sano S . Evidence of increased synthesis of delta-aminolevulinic acid dehydratase in experimental lead-poisoned rats. Biochim Biophys Acta. 1981; 678(1):39-50. DOI: 10.1016/0304-4165(81)90045-3. View

20.

Despaux N, Bohuon C, Comoy E, Boudene C . Postulated mode of action of metals on purified human ALA-dehydratase (EC 4-2-1-24). Biomedicine. 1977; 27(9-10):358-61. View