Functional Heterogeneity of the Descending Limbs of Henle's Loop. II. Interspecies Differences Among Rabbits, Rats, and Hamsters

Permeability properties of the descending limbs of Henle's loop were compared among rabbits, hamsters, and rats by measuring transepithelial voltage (Vt) across the isolated renal tubules perfused in vitro. From the deflection of the Vt when the composition of the bathing fluid was varied, the permeabilities of sodium and of potassium relative to chloride (Pna/PCl and PK/PCl, respectively) were determined in either the descending limbs of the short-loop nephron (SDL) or the segments of the upper protion of the long-loop nephron (LDLu). In hamsters and rats, the values of PNa/PCl of the LDLu (3.98 +/- 0.66 and 5.03 +/- 0.79) were higher than those of the SDL (0.68 +/- 0.03 and 0.61 +/- 0.00). In contrast, in rabbits the value of PNa/PCl of the LDLu (0.96 +/- 0.05) was only slightly higher than that of the SDLu (0.75 +/- 0.03). The similar tendency was also noted in the values of PK/PCl. In hamsters and rats, the PK/PCi ratios were 4.90 +/- 0.82 and 6.44 +/- 0.90, respectively, in the LDLu and 1.09 +/- 0.04 and 1.02 +/- 0.0, respectively in the SDL. When a transepithelial osmotic gradient was imposed by adding raffinose to the bath, a lumen-negative streaming voltage of about -8 mV was generated in the hamster and the rat LDLu. Taken together with the findings in the preceding paper, these observations support the view that the descending limbs of rabbits are different from those of hamsters and rats in that internephron heterogeneity is less remarkable, and that the LDLu of hamsters and rats is highly permeable to sodium and to potassium as well as to water.