Subunit Hybridization and Immunological Studies of Duplicated Phosphoglucose Isomerase Isozymes

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1984 Oct 1

PMID 6517856

Citations 6

Authors

R C Higgins

L D Gottlieb

Affiliations

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Abstract

We examined structural and functional properties of the recently duplicated cytosolic isozymes of phosphoglucose isomerase (PGI) (EC 5.3.1.9) of the wild plant Clarkia xantiana and related species. A new purification protocol yielded PGIs with high specific activities. The duplicated PGI isozymes showed similar substrate affinities (apparent Km) in both catalytic directions. A newly devised competitive enzyme-linked immunosorbent assay (ELISA), using polyclonal antibodies, distinguished sodium dodecyl sulfate (SDS)-denatured PGIs coded by the duplicated loci but did not discriminate between allelic products of one of the loci. In vitro dissociation and reassociation experiments revealed that the duplicated subunits differed in their efficiency of reassociation. The difference was closely correlated with differences between the isozymes in their in vivo accumulation. In contrast, allelic subunits in species with or without the duplication were able to reassociate with similar efficiency. The duplicated PGI isozymes have diverged more in structural properties than kinetic ones. The total evidence suggests that mechanisms have evolved which reduce the potential consequences of the duplication.

Citing Articles

Plant phosphoglucose isomerase genes lack introns and are expressed in Escherichia coli.

Tait R, Froman B, Laudencia-Chingcuanco D, Gottlieb L Plant Mol Biol. 2013; 11(4):381-8.

PMID: 24272394 DOI: 10.1007/BF00039018.

α-1,4-glucan phosphorylase forms from leaves of spinach (Spinacia oleracea L.) : II. Peptide patterns and immunological properties. A comparison with other phosphorylase forms.

Steup M, Schachtele C Planta. 2013; 168(2):222-31.

PMID: 24232025 DOI: 10.1007/BF00402967.

Enzyme activity in ems-induced null mutations of duplicated genes encoding phosphoglucose isomerases in clarkia.

Jones T, Pichersky E, Gottlieb L Genetics. 1986; 113(1):101-14.

PMID: 17246324 PMC: 1202790. DOI: 10.1093/genetics/113.1.101.

Reduced enzyme activity and starch level in an induced mutant of chloroplast phosphoglucose isomerase.

Jones T, Gottlieb L, Pichersky E Plant Physiol. 1986; 81(2):367-71.

PMID: 16664822 PMC: 1075341. DOI: 10.1104/pp.81.2.367.

Molecular characterization of duplicate cytosolic phosphoglucose isomerase genes in Clarkia and comparison to the single gene in Arabidopsis.

Thomas B, Ford V, Pichersky E, Gottlieb L Genetics. 1993; 135(3):895-905.

PMID: 8293986 PMC: 1205728. DOI: 10.1093/genetics/135.3.895.

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