Studies of Triacyglycerol Structure of Very Low Density Lipoproteins of Normolipemic Subjects and Patients with Type III and Type IV Hyperlipoproteinemia

Overview

Journal Lipids

Specialty Biochemistry

Date 1984 Sep 1

PMID 6503630

Citations 3

Authors

J J Myher

A Kuksis

W C Breckenridge

J A Little

Affiliations

Soon will be listed here.

Abstract

The triacylglycerols of very low density lipoproteins (VLDL-TG) were analyzed in samples from normal subjects and patients with Frederickson's Type III and Type IV hyperlipoproteinemia. VLDL were obtained by conventional ultracentrifugation, and the triacylglycerols were isolated by thin-layer chromatography (TLC). Representative sn-1,2(2,3)- and sn-1,3-diacylglycerols were generated by Grignard degradation of the triacylglycerols, and were resolved by TLC on borate-treated silica gel. The molecular association of the fatty acids in the diacylglycerol moieties was determined by gas-liquid chromatography with mass spectrometry (GC/MS) of the tertiary-butyldimethylsilyl ethers. The positional distribution of the fatty acids was established by the Brockerhoff stereospecific analysis. The results showed a marked asymmetry in the distribution of the fatty acids in all samples, with the saturated acids predominantly in the sn-1-position and the unsaturated fatty acids distributed about equally between the sn-2- and sn-3-positions. In all instances, the molecular species composition of the sn-1,2-, sn-2,3- and sn-1,3-diacylglycerols was found to be similar to that calculated for 1-random 2-random 3-random distribution of triacylglycerols. There were marked differences in the quantitative composition of the molecular species of the VLDL-TG between normal subjects and patients, but these discrepancies were attributed to differences in the fatty acid composition of the samples.

Citing Articles

Effect of triacylglycerol structure on absorption and metabolism of isotope-labeled palmitic and linoleic acids by humans.

Emken E, Adlof R, Duval S, Shane J, Walker P, Becker C Lipids. 2004; 39(1):1-9.

PMID: 15055228 DOI: 10.1007/s11745-004-1194-6.

Comparative studies of triacylglycerol structure of very low density lipoproteins and chylomicrons of normolipemic subjects and patients with type II hyperlipoproteinemia.

Myher J, Kuksis A, Breckenridge W, McGuire V, Little J Lipids. 1985; 20(2):90-101.

PMID: 3982238 DOI: 10.1007/BF02534214.

Fatty acid composition of individual plasma steryl esters in phytosterolemia and xanthomatosis.

Kuksis A, Myher J, Marai L, Little J, McArthur R, RONCARI D Lipids. 1986; 21(6):371-7.

PMID: 3736345 DOI: 10.1007/BF02534930.

References

Jensen R, Clark R, Gerrior S, Fey M, Gotto Jr A . Cholesteryl ester and triacylglycerol fatty acids in type V hyperlipidemia. Lipids. 1979; 14(7):691-4. DOI: 10.1007/BF02533457. View

Hatch F . Practical methods for plasma lipoprotein analysis. Adv Lipid Res. 1968; 6:1-68. View

Brockerhoff H . A STEREOSPECIFIC ANALYSIS OF TRIGLYCERIDES. J Lipid Res. 1965; 6:10-5. View

PARIJS J, DE WEERDT G, Beke R, Barbier F . Stereospecific distribution of fatty acids in human plasma triglycerides. Clin Chim Acta. 1976; 66(1):43-52. DOI: 10.1016/0009-8981(76)90369-7. View

PARIJS J, DE WEERDT G, Beke R, Barbier F . Stereospecific analysis of human plasma triglycerides. Lipids. 1974; 9(11):937-8. DOI: 10.1007/BF02532622. View

Kuksis A, Myher J, GEHER K, Hoffman A, Breckenridge W, Jones G . Comparative determination of plasma cholesterol and triacylglycerol levels by automated gas--liquid chromatographic and autoanalyzer methods. J Chromatogr. 1978; 146(3):393-412. DOI: 10.1016/s0378-4347(00)81198-6. View

Morley N, Kuksis A, Buchnea D . Hydrolysis of synthetic triacylglycerols by pancreatic and lipoprotein lipase. Lipids. 1974; 9(7):481-8. DOI: 10.1007/BF02534275. View

Beil U, Grundy S, Crouse J, ZECH L . Triglyceride and cholesterol metabolism in primary hypertriglyceridemia. Arteriosclerosis. 1982; 2(1):44-57. DOI: 10.1161/01.atv.2.1.44. View

Myher J, Marai L, Kuksis A . Identification of fatty acids by GC-MS using polar siloxane liquid phases. Anal Biochem. 1974; 62(1):188-203. DOI: 10.1016/0003-2697(74)90380-7. View

10.

Gordon D, Pitas R, Jensen R . Effects of diet and type IIa hyperlipoproteinemia upon structure of triacylglycerols and phosphatidyl cholines from human plasma lipoproteins. Lipids. 1975; 10(5):270-83. DOI: 10.1007/BF02532700. View

11.

Kuksis A, Marai L, Myher J . Triglyceride structure of milk fats. J Am Oil Chem Soc. 1973; 50(6):193-201. DOI: 10.1007/BF02640489. View

12.

Myher J, Marai L, Kuksis A . Acylglycerol structure of peanut oils of different atherogenic potential. Lipids. 1977; 12(10):775-85. DOI: 10.1007/BF02533264. View

13.

Christie W, Moore J . A comparison of the structures of triglycerides from various pig tissues. Biochim Biophys Acta. 1970; 210(1):46-56. DOI: 10.1016/0005-2760(70)90060-3. View

14.

Lands W, Pieringer R, Slakey S, Zschocke A . A micromethod for the stereospecific determination of triglyceride structure. Lipids. 1966; 1(6):444-8. DOI: 10.1007/BF02532550. View

15.

Breckenridge W, Yeung S, Kuksis A, Myher J, Chan M . Biosynthesis of diacylglycerols by rat intestinal mucosa in vivo. Can J Biochem. 1976; 54(2):137-44. DOI: 10.1139/o76-021. View

16.

Bugaut M, Myher J, Kuksis A, Hoffman A . An examination of the stereochemical course of acylation of 2-monoacylglycerols by rat intestinal villus cells using [2H3]palmitic acid. Biochim Biophys Acta. 1984; 792(3):254-69. DOI: 10.1016/0005-2760(84)90193-0. View

17.

Yamashita S, Hosaka K, Numa S . Acyl-donor specificities of partially purified 1-acylglycerophosphate acyltransferase, 2-acylglycerophosphate acyltransferase and 1-acylglycerophosphorylcholine acyltransferase from rat-liver microsomes. Eur J Biochem. 1973; 38(1):25-31. DOI: 10.1111/j.1432-1033.1973.tb03028.x. View

18.

Allard C, Davignon J, Marcel Y, Goulet C, Kuba K, Alteresco M . Fatty acid profile of plasma lipid classes in coronary heart disease, in primary hyperlipoproteinemias, and in healthy subjects. Can J Biochem. 1973; 51(11):1509-14. DOI: 10.1139/o73-200. View

19.

Folch J, Lees M, SLOANE STANLEY G . A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957; 226(1):497-509. View

20.

Yurkowski M, Brockerhoff H . Fatty acid distribution of triglycerides determined by deacylation with methyl magnesium bromide. Biochim Biophys Acta. 1966; 125(1):55-9. DOI: 10.1016/0005-2760(66)90143-3. View