In Vitro Labeling of Human Polymorphonuclear Leukocytes with 99mTc

Overview

Journal Eur J Nucl Med

Specialty Nuclear Medicine

Date 1984 Jan 1

PMID 6489369

Citations 3

Authors

H Kelbaek

J Fogh

J Elmgreen

Affiliations

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Abstract

Preliminary experience with 99mTc labeling of isolated polymorphonuclear leukocytes is reported. Leukocytes efficiently purified from 100 ml peripheral blood were labeled in vitro with 99mTc after pretreatment with small amounts of a stannous agent to reduce pertechnetate. A labeling yield of 1.3 mCi equivalent to 12.5% of the added dose of 99mTc and a cell binding efficiency of more than 90% was achieved under sterile conditions. Viability assays showed no influence of the labeling procedure on cell function. The technique may prove a valuable alternative for assessment of local leukocyte accumulation by scintigraphy.

Citing Articles

Scintigraphic demonstration of subcutaneous abscesses with 99mTc-labeled leukocytes.

Kelbaek H, Fogh J, Gjorup T, BULOW K, Vestergaard B Eur J Nucl Med. 1985; 10(7-8):302-3.

PMID: 4006973 DOI: 10.1007/BF00251300.

Isolation and labelling of human leukocytes with 99mTc.

Kelbaek H Eur J Nucl Med. 1986; 12(3):107-9.

PMID: 3758100 DOI: 10.1007/BF00276700.

Evaluation of a new leukocyte labeling procedure with 99mTc-HMPAO.

Kelbaek H, Linde J, Nielsen S Eur J Nucl Med. 1988; 14(12):621-3.

PMID: 3243309 DOI: 10.1007/BF00251786.

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