An Automated "high-pressure" Liquid-chromatographic Assay for Hemoglobin A1c

Overview

Journal Clin Chem

Publisher Oxford University Press

Specialty Biochemistry

Date 1984 Nov 1

PMID 6488517

Citations 17

Authors

G Ellis

E P Diamandis

E E Giesbrecht

D Daneman

L C Allen

Affiliations

Soon will be listed here.

Abstract

An automated "high-pressure" liquid-chromatographic assay for hemoglobin A1c is described. We use a 45-min incubation in acetate buffer (pH 5.5) to eliminate labile glycated hemoglobins. In this automated system conventional modules are used but it incorporates a solvent-switching valve to select either of two buffers, which differ in pH and NaCl concentration. The chromatographic column contains "polyCAT" (a weak cation-exchanger, polyaspartic acid linked to silica). Run time is 14 min per sample. The method is precise and results correlate well with those by other ion-exchange procedures.

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