Interaction Between Insulin Receptors and Major Histocompatibility Complex Antigens in Mouse Liver Membranes

Overview

Journal Diabetologia

Specialty Endocrinology

Date 1984 Jul 1

PMID 6479491

Citations 3

Authors

N Brossette

E Van Obberghen

M Fehlmann

Affiliations

Soon will be listed here.

Abstract

Insulin receptors from H-2k mouse liver membranes were specifically labelled using a photoreactive insulin analogue coupled to the alpha-subunit (Mr 130,000) of the receptor by ultraviolet-irradiation. Up to 25% of the labelled insulin receptors recovered after membrane solubilization and precipitable by anti-insulin receptor antibodies could be immunoprecipitated by various monoclonal antibodies reacting with H-2Kk antigens. None of these monoclonal antibodies inhibited insulin binding to its receptor. These results show that insulin receptors and H-2Kk antigens interact in liver plasma membranes to form non-covalent complexes. This association between insulin receptors and major histocompatibility complex antigens could be involved in the pathogenesis of certain forms of insulin resistance.

Citing Articles

Molecular association between major histocompatibility complex class I antigens and insulin receptors in mouse liver membranes.

Fehlmann M, Peyron J, Samson M, Van Obberghen E, BRANDENBURG D, Brossette N Proc Natl Acad Sci U S A. 1985; 82(24):8634-7.

PMID: 3866245 PMC: 391490. DOI: 10.1073/pnas.82.24.8634.

The structure of the hepatic insulin receptor and insulin binding.

Haynes F, Helmerhorst E, Yip C Biochem J. 1986; 239(1):127-33.

PMID: 3026365 PMC: 1147249. DOI: 10.1042/bj2390127.

Photoaffinity labelling of hepatic plasma membranes suggests two classes of hepatic insulin receptor.

Haynes F, Yip C Diabetologia. 1985; 28(10):786-92.

PMID: 2998914 DOI: 10.1007/BF00265029.

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