Nuclear Magnetic Resonance Study of D-TGGCCA in Solution

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1984 Jul 25

PMID 6462922

Citations 3

Authors

E Guittet

D Piveteau

J Y Lallemand

J IGOLEN

Affiliations

Soon will be listed here.

Abstract

The 1H NMR spectrum of a self complementary deoxyhexanucleotide (d-TGGCCA) in water solution has been completely and unambiguously assigned by use of two-dimensional techniques and phosphorus off-resonance decoupling experiments. The helix-coil transition has been monitored by following most of the non-exchangeable protons against temperature and was shown to be a cooperative dissociation-association process. Sequential fraying is sizable even at low temperature. Considerations of coupling constants and of Nuclear Overhauser effects show the helix to be of the B-DNA type.

Citing Articles

2D-NMR studies of the unnatural duplex alpha-d(TCTAAAC)-beta-d(AGATTTG).

Lancelot G, Guesnet J, Roig V, Thuong N Nucleic Acids Res. 1987; 15(18):7531-47.

PMID: 3658702 PMC: 306266. DOI: 10.1093/nar/15.18.7531.

alpha-DNA-III. Characterization by high field 1H-NMR, anti-parallel self-recognition and conformation of the unnatural hexadeoxyribonucleotides alpha-[d(CpApTpGpCpG)] and alpha-[d(CpGpCpApTpG)]. Alpha-oligodeoxynucleotides as potential cellular....

Morvan F, Rayner B, Imbach J, Chang D, Lown J Nucleic Acids Res. 1987; 15(10):4241-55.

PMID: 3588292 PMC: 340845. DOI: 10.1093/nar/15.10.4241.

Study of structure, base-pair opening kinetics and proton exchange mechanism of the d-(AATTGCAATT) self-complementary oligodeoxynucleotide in solution.

Kochoyan M, Lancelot G, Leroy J Nucleic Acids Res. 1988; 16(15):7685-702.

PMID: 2842732 PMC: 338435. DOI: 10.1093/nar/16.15.7685.

References

Remin M, SHUGAR D . Conformation of the exocyclic 5'-CH 2 OH in nucleosides and nucleotides in aqueous solution from specific assignments of the H 5' and H 5'' signals in the NMR spectra. Biochem Biophys Res Commun. 1972; 48(3):636-42. DOI: 10.1016/0006-291x(72)90395-6. View

Itakura K, Katagiri N, Bahl C, Wightman R, Narang S . Improved triester approach for the synthesis of pentadecathymidylic acid. J Am Chem Soc. 1975; 97(25):7327-32. DOI: 10.1021/ja00858a020. View

Nagayama K, Wuthrich K, Ernst R . Two-dimensional spin echo correlated spectroscopy (SECSY) for 1H NMR studies of biological macromolecules. Biochem Biophys Res Commun. 1979; 90(1):305-11. DOI: 10.1016/0006-291x(79)91625-5. View

Patel D, Kozlowski S, Marky L, Broka C, Rice J, Itakura K . Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solution. Biochemistry. 1982; 21(3):428-36. DOI: 10.1021/bi00532a002. View

Patel D, Kozlowski S, Nordheim A, Rich A . Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR. Proc Natl Acad Sci U S A. 1982; 79(5):1413-7. PMC: 345983. DOI: 10.1073/pnas.79.5.1413. View

Tran-Dinh S, Neumann J, Huynh-Dinh T, Allard P, Lallemand J, IGOLEN J . DNA fragment conformations IV - Helix-coil transition and conformation of d-CCATGG in aqueous solution by 1H-NMR spectroscopy. Nucleic Acids Res. 1982; 10(17):5319-32. PMC: 320874. DOI: 10.1093/nar/10.17.5319. View

Hare D, Wemmer D, Chou S, Drobny G, Reid B . Assignment of the non-exchangeable proton resonances of d(C-G-C-G-A-A-T-T-C-G-C-G) using two-dimensional nuclear magnetic resonance methods. J Mol Biol. 1983; 171(3):319-36. DOI: 10.1016/0022-2836(83)90096-7. View

Petersheim M, Turner D . Nuclear overhauser studies of CCGGAp, ACCGGp, and ACCGGUp. Biochemistry. 1983; 22(2):264-8. DOI: 10.1021/bi00271a005. View

Petersheim M, Turner D . Proton magnetic resonance melting studies of CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp. Biochemistry. 1983; 22(2):269-77. DOI: 10.1021/bi00271a006. View

10.

Girault J, Chottard J, Guittet E, Lallemand J, Huynh-Dinh T, IGOLEN J . Specific platinum chelation by the guanines of the deoxyhexanucleotide d(T-G-G-C-C-A) upon reaction with cis-[Pt(NH3)2(H2O)2](NO3)2. Biochem Biophys Res Commun. 1982; 109(4):1157-63. DOI: 10.1016/0006-291x(82)91898-8. View

11.

Reid D, Salisbury S, Bellard S, Shakked Z, Williams D . Proton nuclear Overhauser effect study of the structure of a deoxyoligonucleotide duplex in aqueous solution. Biochemistry. 1983; 22(8):2019-25. DOI: 10.1021/bi00277a044. View

12.

Lankhorst P, Wille G, VAN Boom J, Altona C, Haasnoot C . Conformational analysis of a ribopentanucleoside tetraphosphate in aqueous solution. A two-dimensional NMR study at 500 MHz. Nucleic Acids Res. 1983; 11(9):2839-56. PMC: 325927. DOI: 10.1093/nar/11.9.2839. View

13.

Frechet D, Cheng D, Kan L, Tso P . Nuclear Overhauser effect as a tool for the complete assignment of nonexchangeable proton resonances in short deoxyribonucleic acid helices. Biochemistry. 1983; 22(22):5194-200. DOI: 10.1021/bi00291a020. View

14.

Petersheim M, Turner D . Base-stacking and base-pairing contributions to helix stability: thermodynamics of double-helix formation with CCGG, CCGGp, CCGGAp, ACCGGp, CCGGUp, and ACCGGUp. Biochemistry. 1983; 22(2):256-63. DOI: 10.1021/bi00271a004. View