Strains of Escherichia Coli Carrying the Structural Gene for Histidyl-tRNA Synthetase on a High Copy-number Plasmid

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1981 Jan 1

PMID 6460151

Citations 14

Authors

S J Eisenbeis

J Parker

Affiliations

Soon will be listed here.

Abstract

That portion of the Escherichia coli chromosome carried by a number of lambda transducing phages, all of which carry the gua operon, was mapped using restriction endonucleases. The DNA from one of these transducing phages was subcloned onto pBR322. We have identified two recombinant plasmids which carry the Escherichia coli gene hisS, the structural gene for histidyl-tRNA synthetase. The two plasmids, pSE301 and pSE401, have in common a 3,540 bp fragment of E. coli DNA which is bounded by BglII and SalI restriction endonuclease recognition sites. Strains carrying these plasmids overproduce histidyl-tRNA synthetase 20 to 30 fold. The growth rate of these strains is not affected although the histidine biosynthetic enzymes are derepressed. This derepression seems to be in addition to that caused by introduction of a hisT mutation on the chromosome.

Citing Articles

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

Berlyn M Microbiol Mol Biol Rev. 1998; 62(3):814-984.

PMID: 9729611 PMC: 98936. DOI: 10.1128/MMBR.62.3.814-984.1998.

aarC, an essential gene involved in density-dependent regulation of the 2'-N-acetyltransferase in Providencia stuartii.

Rather P, Solinsky K, Paradise M, Parojcic M J Bacteriol. 1997; 179(7):2267-73.

PMID: 9079912 PMC: 178963. DOI: 10.1128/jb.179.7.2267-2273.1997.

Overproduction of tryptophanyl-tRNA synthetase relieves transcription termination at the Escherichia coli tryptophan operon attenuator.

Das A, Yanofsky C J Bacteriol. 1984; 160(2):805-7.

PMID: 6389500 PMC: 214812. DOI: 10.1128/jb.160.2.805-807.1984.

Isolation and characterization of regulatory mutations affecting the expression of the guaBA operon of Escherichia coli K-12.

Tiedeman A, Smith J Mol Gen Genet. 1984; 195(1-2):77-82.

PMID: 6387393 DOI: 10.1007/BF00332727.

Identification of the purC gene product of Escherichia coli.

Parker J J Bacteriol. 1984; 157(3):712-7.

PMID: 6365889 PMC: 215316. DOI: 10.1128/jb.157.3.712-717.1984.

References

Pedersen S, Bloch P, Reeh S, Neidhardt F . Patterns of protein synthesis in E. coli: a catalog of the amount of 140 individual proteins at different growth rates. Cell. 1978; 14(1):179-90. DOI: 10.1016/0092-8674(78)90312-4. View

Bruni C, Colantuoni V, Sbordone L, Cortese R, Blasi F . Biochemical and regulatory properties of Escherichia coli K-12 hisT mutants. J Bacteriol. 1977; 130(1):4-10. PMC: 235167. DOI: 10.1128/jb.130.1.4-10.1977. View

Bloch P, Phillips T, Neidhardt F . Protein identifications of O'Farrell two-dimensional gels: locations of 81 Escherichia coli proteins. J Bacteriol. 1980; 141(3):1409-20. PMC: 293839. DOI: 10.1128/jb.141.3.1409-1420.1980. View

Szybalski E, SZYBALSKI W . A comprehensive molecular map of bacteriophage lambda. Gene. 1979; 7(3-4):217-70. DOI: 10.1016/0378-1119(79)90047-7. View

Williams L, Neidhardt F . Synthesis and inactivation of aminoacyl-transfer RNA synthetases during growth of Escherichia coli. J Mol Biol. 1969; 43(3):529-50. DOI: 10.1016/0022-2836(69)90357-x. View

Johnston H, Barnes W, Chumley F, Bossi L, Roth J . Model for regulation of the histidine operon of Salmonella. Proc Natl Acad Sci U S A. 1980; 77(1):508-12. PMC: 348301. DOI: 10.1073/pnas.77.1.508. View

NASS G . Regulation of histidine biosynthetic enzymes in a mutant of Escherichia coli with an altered histidyl-tRNA synthetase. Mol Gen Genet. 1967; 100(2):216-24. DOI: 10.1007/BF00333608. View

Lawther R, Hatfield W . Biochemical characterization of an Escherichia coli hisT strain. J Bacteriol. 1977; 130(1):552-7. PMC: 235243. DOI: 10.1128/jb.130.1.552-557.1977. View

Parker J, Pollard J, Friesen J, Stanners C . Stuttering: high-level mistranslation in animal and bacterial cells. Proc Natl Acad Sci U S A. 1978; 75(3):1091-5. PMC: 411414. DOI: 10.1073/pnas.75.3.1091. View

10.

Plumbridge J, Springer M . Genes for the two subunits of phenylalanyl-tRNA synthesis of Escherichia coli are transcribed from the same promoter. J Mol Biol. 1980; 144(4):595-600. DOI: 10.1016/0022-2836(80)90341-1. View

11.

Bachmann B, Low K . Linkage map of Escherichia coli K-12, edition 6. Microbiol Rev. 1980; 44(1):1-56. PMC: 373233. DOI: 10.1128/mr.44.1.1-56.1980. View

12.

Chapeville F, LIPMANN F, VON EHRENSTEIN G, Weisblum B, Ray Jr W, Benzer S . On the role of soluble ribonucleic acid in coding for amino acids. Proc Natl Acad Sci U S A. 1962; 48:1086-92. PMC: 220908. DOI: 10.1073/pnas.48.6.1086. View

13.

Parker J, Fishman S . Mapping hisS, the structural gene for histidyl-transfer ribonucleic acid synthetase, in Escherichia coli. J Bacteriol. 1979; 138(1):264-7. PMC: 218269. DOI: 10.1128/jb.138.1.264-267.1979. View

14.

McGinnis E, Williams L . Regulation of histidyl-transfer ribonucleic acid synthetase formation in a histidyl-transfer ribonucleic acid synthetase mutant of Salmonella typhimurium. J Bacteriol. 1972; 111(3):739-44. PMC: 251347. DOI: 10.1128/jb.111.3.739-744.1972. View

15.

Neidhardt F, Bloch P, Pedersen S, Reeh S . Chemical measurement of steady-state levels of ten aminoacyl-transfer ribonucleic acid synthetases in Escherichia coli. J Bacteriol. 1977; 129(1):378-87. PMC: 234936. DOI: 10.1128/jb.129.1.378-387.1977. View

16.

Murray M, Hartman P . Overproduction of hisH and hisF gene products leads to inhibition of cell cell division in Salmonella. Can J Microbiol. 1972; 18(5):671-81. DOI: 10.1139/m72-105. View

17.

Ofarrell P . The suppression of defective translation by ppGpp and its role in the stringent response. Cell. 1978; 14(3):545-57. DOI: 10.1016/0092-8674(78)90241-6. View

18.

Barnes W . DNA sequence from the histidine operon control region: seven histidine codons in a row. Proc Natl Acad Sci U S A. 1978; 75(9):4281-5. PMC: 336097. DOI: 10.1073/pnas.75.9.4281. View

19.

Di Nocera P, Blasi F, Di Lauro R, Frunzio R, Bruni C . Nucleotide sequence of the attenuator region of the histidine operon of Escherichia coli K-12. Proc Natl Acad Sci U S A. 1978; 75(9):4276-80. PMC: 336096. DOI: 10.1073/pnas.75.9.4276. View

20.

Clewell D, Helinski D . Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event. Biochemistry. 1970; 9(22):4428-40. DOI: 10.1021/bi00824a026. View