[Mapping of Mutations Within the Genes Coding for Enzyme I and Hpr Proteins of the Phosphoenolpyruvate-dependent Phosphotransferase System of Escherichia Coli K-12. I. Mapping of the Mutations Within the PtsI Gene]

Fine genetic mapping of the pts region coding for general components of PTS was performed. Over 30 spontaneous pts mutations were investigated. By means of the complementation test using the F'trp+ purC+ ptsI episome, both ptsI and ptsH mutations were revealed among them. With the help of reciprocal three point transduction crosses, 8 of them were situated in the pts region. Two of them were in ptsH gene, the rest being in ptsI gene. The lysogenic strain was obtained with lambda inserted in the pts region. Heat curing of the lysogene led to a number of deletions and insertions. Six of them were mapped with the help of the point mutations studied.