Partial Characterization of the Plasma Membrane ATPase from a Rho0 Petite Strain of Saccharomyces Cerevisiae

Overview

Journal J Bioenerg Biomembr

Publisher Springer

Specialties Biochemistry
Biology
Endocrinology

Date 1980 Aug 1

PMID 6452450

Citations 2

Authors

J P McDonough

P K Jaynes

H R Mahler

Affiliations

Soon will be listed here.

Abstract

Crude membrane preparations of a rho0 mutant of Saccharomyces cerevisiae exhibit Mg2+-dependent ATPase activity. Over the optimal pH range, 5.0-6.75, the apparent Vmax of the enzyme equals 590 nmoles of ATP hydrolyzed per minute per milligram protein, with an apparent Km for ATP of 1.3 mM. ATP hydrolysis is insensitive to ouabain, venturicidin, aurovertin, and the protein inhibitor described by Pullman and Monroy; inhibited by oligomycin (at high concentrations) and sodium orthovanadate, and it is sensitive to dicyclohexylcarbodiimide, p-hydroxymercuribenzoate, hydroxylamine, sodium fluoride, and sodium iodoacetate. The pH optimum and the inhibitor pattern distinguish the plasma membrane enzyme from the mitochondrial F1 ATPase still present in these cells (this activity is sensitive to efrapeptin, aurovertin, and the protein inhibitor, but resistant to DCCD). In addition, the activity of the plasma membrane enzyme and its affinity for ATP are responsive to changes in the composition of the growth medium, with the highest activity observed in cells grown on methyl-alpha-D-glucoside, a sugar which results not only in partial release from catabolite repression but also requires the induction of an active transport system for growth.

Citing Articles

Properties and possible functions of the adenylate cyclase in plasma membranes of Saccharomyces cerevisiae.

Jaynes P, McDonough J, Mahler H Mol Cell Biol. 1982; 2(12):1481-91.

PMID: 14582190 PMC: 369957. DOI: 10.1128/mcb.2.12.1481-1491.1982.

Two distinct subfractions in isolated Saccharomyces cerevisiae plasma membranes.

Tschopp J, Schekman R J Bacteriol. 1983; 156(1):222-9.

PMID: 6137475 PMC: 215073. DOI: 10.1128/jb.156.1.222-229.1983.

References

FUHRMANN G, Boehm C, Theuvenet A . Sugar transport and potassium permeability in yeast plasma membrane vesicles. Biochim Biophys Acta. 1976; 433(3):583-96. DOI: 10.1016/0005-2736(76)90283-2. View

Altendorf K . Purification of the DCCD-reactive protein of the energy-transducing adenosine triphosphatase complex from Escherichia coli. FEBS Lett. 1977; 73(2):271-5. DOI: 10.1016/0014-5793(77)80997-6. View

Schatz G . Impaired binding of mitochondrial adenosine triphosphatase in the cytoplasmic "petite" mutant of Saccharomyces cerevisiae. J Biol Chem. 1968; 243(9):2192-9. View

FUHRMANN G, WEHRLI E, Boehm C . Preparation and identification of yeast plasma membrane vesicles. Biochim Biophys Acta. 1974; 363(3):295-310. DOI: 10.1016/0005-2736(74)90070-4. View

Foury F, Boutry M, Goffeau A . Efflux of potassium induced by dio-9, a plasma membrane ATPase inhibitor in the yeast Schizosaccharomyces pombe. J Biol Chem. 1977; 252(13):4577-83. View

Tzagoloff A, Rubin M, Sierra M . Biosynthesis of mitochondrial enzymes. Biochim Biophys Acta. 1973; 301(1):71-104. DOI: 10.1016/0304-4173(73)90013-x. View

RIEMERSMA J, Alsbach E . Proton translocation during anaerobic energy production in Saccharomyces cerevisiae. Biochim Biophys Acta. 1974; 339(2):274-84. DOI: 10.1016/0005-2736(74)90324-1. View

Dianoux A, Bof M, Vignais P . The dicyclohexylcarbodiimide-binding protein of rat liver mitochondria as a product of the mitochondrial protein synthesis. Eur J Biochem. 1978; 88(1):69-77. DOI: 10.1111/j.1432-1033.1978.tb12423.x. View

RACKER E . Reconstitution of cytochrome oxidase vesicles and conferral of sensitivity to energy transfer inhibitors. J Membr Biol. 1972; 10(3):221-35. DOI: 10.1007/BF01867856. View

10.

Bowman B, SLAYMAN C . The effects of vanadate on the plasma membrane ATPase of Neurospora crassa. J Biol Chem. 1979; 254(8):2928-34. View

11.

WEIBULL C, GREENWALT J, Low H . The hydrolysis of adenosine triphosphate by cell fractions of Bacillus megaterium. I. Localization and general characteristics of the enzymic activities. J Biol Chem. 1962; 237:847-52. View

12.

Whittam R, WHEELER K, Blake A . OLIGOMYCIN AND ACTIVE TRANSPORT REACTIONS IN CELL MEMBRANES. Nature. 1964; 203:720-4. DOI: 10.1038/203720a0. View

13.

Susa J, LARDY H . Antibiotics as tools for metabolic studies. XVIII. Inhibition of sodium- and potassium-dependent adenosine triphosphatase. Mol Pharmacol. 1975; 11(2):166-73. View

14.

Cantley Jr L, Josephson L, Warner R, Yanagisawa M, Lechene C, Guidotti G . Vanadate is a potent (Na,K)-ATPase inhibitor found in ATP derived from muscle. J Biol Chem. 1977; 252(21):7421-3. View

15.

Brocklehurst R, Gardner D, Eddy A . The absorption of protons with alpha-methyl glucoside and alpha-thioethyl glucoside by the yeast N.C.Y.C. 240. Evidence against the phosphorylation hypothesis. Biochem J. 1977; 162(3):591-9. PMC: 1164642. DOI: 10.1042/bj1620591. View

16.

Kovac L . Biochemical mutants: an approach to mitochondrial energy coupling. Biochim Biophys Acta. 1974; 346(2):101-35. View

17.

Cockburn M, Earnshaw P, Eddy A . The stoicheiometry of the absorption of protons with phosphate and L-glutamate by yeasts of the genus Saccharomyces. Biochem J. 1975; 146(3):705-12. PMC: 1165361. DOI: 10.1042/bj1460705. View

18.

Simons T . Vanadate--a new tool for biologists. Nature. 1979; 281(5730):337-8. DOI: 10.1038/281337a0. View

19.

HINKLE P, Horstman L . Respiration-driven proton transport in submitochondrial particles. J Biol Chem. 1971; 246(19):6024-8. View

20.

Scarborough G . Properties of Neurospora crassa plasma membrane ATPase. Arch Biochem Biophys. 1977; 180(2):384-93. DOI: 10.1016/0003-9861(77)90052-2. View