W-reactivation of Phage Lambda in X-irradiated Mutants of Escherichia Coli K-12

Overview

Journal Radiat Environ Biophys

Specialties Biophysics
Environmental Health
Oncology
Radiology

Date 1980 Jan 1

PMID 6449715

Citations 2

Authors

K D Martignoni

I Haselbacher

Affiliations

Soon will be listed here.

Abstract

The survival of UV irradiated phage lambda was increased on X-irradiated E. coli K-12 host cells over that on unirradiated cells. The frequency of c mutants among the surviving phages was to a similar extent increased by the X-ray exposure of the host cells as by UV light. This W-reactivation of phage lambda occurred in uvrA, polA, and recB mutants besides the wild type at about equal X-ray doses, however, at a reduced reactivation efficiency compared with the wild type. W-reactivation was undetectable in recA mutants. While maximal UV induced W-reactivation occurred 30 min after irradiation, the maximal X-ray induced reactivation was found immediately after irradiation. Chloramphenicol (100 micrograms/ml) and nitrofurantoin (50 micrograms/ml) inhibited W-reactivation of phage lambda if added before irradiation of the host cells, indicating the necessity of protein synthesis for W-reactivation.

Citing Articles

Mutagenesis of lambda phage: weigle mutagenesis is induced by coincident lesions in the double helical DNA of the host cell genome.

Hutchinson F, Stein J Mol Gen Genet. 1981; 181(4):458-63.

PMID: 6455586 DOI: 10.1007/BF00428736.

Changes in DNA base sequence induced by gamma-ray mutagenesis of lambda phage and prophage.

Tindall K, Stein J, Hutchinson F Genetics. 1988; 118(4):551-60.

PMID: 2966755 PMC: 1203310. DOI: 10.1093/genetics/118.4.551.

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