Cytoplasmic Membrane Proteins of Spectinomycin-susceptible and -resistant Strains of Neisseria Gonorrhoeae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1980 Jul 1

PMID 6447142

Citations 1

Authors

M A Miller

C S Scharli

J Mills

Affiliations

Soon will be listed here.

Abstract

Cytoplasmic membranes were isolated and examined from two spectinomycin-susceptible and three spectinomycin-resistant clinical strains of Neisseria gonorrhoeae. A laboratory-derived spectinomycin-resistant mutant, obtained by serial passage on gradually increasing concentrations of the antibiotic, and a susceptible revertant, spontaneously arising from one of the resistant clinical strains, were also studied. Sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis revealed that a major protein, comprising about 7% of total cytoplasmic membrane protein (molecular weight 24,000), was absent in the three clinically isolated spectinomycin-resistant strains. In a revertant, this protein reappeared. During treatment of one of the susceptible strains with spectinomycin, the protein disappeared. However, this correlation was not maintained in the laboratory-derived spectinomycin-resistant mutant. This mutant was of comparable resistant to the clinical isolates, but the 24,000-molecular-weight protein was present in normal quantities. In addition, spectinomycin resistant in clinical isolates was variable compared with stable resistance exhibited by the laboratory-derived mutant. These findings suggested that differences in laboratory-derived versus clinical spectinomycin resistance may be due to different types of resistance mutations.

Citing Articles

Inhibition of Neisseria gonorrhoeae isolates by Martin-Lewis medium. Epidemiology, susceptibility profile, and plasma analysis.

Miller M, Anderson P, Parker J, ROHRER H Br J Vener Dis. 1982; 58(2):96-100.

PMID: 6802441 PMC: 1046015. DOI: 10.1136/sti.58.2.96.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Birge E, Kurland C . Reversion of a streptomycin-dependent strain of Escherichia coli. Mol Gen Genet. 1970; 109(4):356-69. DOI: 10.1007/BF00267704. View

Osborn M, Gander J, Parisi E, Carson J . Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane. J Biol Chem. 1972; 247(12):3962-72. View

Hill J, Peterson N, Weiss E . Characterization of spheroplast membranes of Neisseria meningitidis group B. Infect Immun. 1972; 5(4):612-21. PMC: 422412. DOI: 10.1128/iai.5.4.612-621.1972. View

REYN A, Schmidt H, TRIER M, Bentzon M . Spectinomycin hydrochloride (Trobicin) in the treatment of gonorrhoea. Observation of resistant strains of Neisseria gonorrhoeae. Br J Vener Dis. 1973; 49(1):54-9. PMC: 1044851. DOI: 10.1136/sti.49.1.54. View

Hasenbank R, Guthrie C, Stoffler G, WITTMANN H, Rosen L, Apirion D . Electrophoretic and immunological studies on ribosomal proteins of 100 Escherichia coli revertants from streptomycin dependence. Mol Gen Genet. 1973; 127(1):1-18. DOI: 10.1007/BF00267778. View

Washington JA I, Yu P . In vitro antibacterial activity of spectinomycin. Antimicrob Agents Chemother. 1972; 2(6):427-30. PMC: 444334. DOI: 10.1128/AAC.2.6.427. View

Johnston K, Gotschlich E . Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol. 1974; 119(1):250-7. PMC: 245596. DOI: 10.1128/jb.119.1.250-257.1974. View

Sarubbi Jr F, Blackman E, Sparling P . Genetic mapping of linked antibiotic resistance loci in Neisseria gonorrhoeae. J Bacteriol. 1974; 120(3):1284-92. PMC: 245913. DOI: 10.1128/jb.120.3.1284-1292.1974. View

10.

Maness M, FOSTER G, Sparling P . Ribosomal resistance to streptomycin and spectinomycin in Neisseria gonorrhoeae. J Bacteriol. 1974; 120(3):1293-9. PMC: 245914. DOI: 10.1128/jb.120.3.1293-1299.1974. View

11.

Miller M, Kuemmerle N, Gentile G . Amoxycillin and ampicillin. A comparative study of in vitro sensitivity and induced morphological alterations in Serratia marcescens. Jpn J Microbiol. 1975; 19(3):219-24. View

12.

Johnston K, Holmes K, Gotschlich E . The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J Exp Med. 1976; 143(4):741-58. PMC: 2190160. DOI: 10.1084/jem.143.4.741. View

13.

Frasch C, McNelis R, Gotschlich E . Strain-specific variation in the protein and lipopolysaccharide composition of the group B meningococcal outer membrane. J Bacteriol. 1976; 127(2):973-81. PMC: 233007. DOI: 10.1128/jb.127.2.973-981.1976. View

14.

Mizuno T, Yamagata H, Mizushima S . Interaction of cytoplasmic membrane and ribosomes in Escherichia coli: spectinomycin-induced disappearance of membrane protein I-19. J Bacteriol. 1977; 129(1):326-32. PMC: 234930. DOI: 10.1128/jb.129.1.326-332.1977. View

15.

Walstad D, Guymon L, Sparling P . Altered outer membrane protein in different colonial types of Neisseria gonorrhoeae. J Bacteriol. 1977; 129(3):1623-7. PMC: 235143. DOI: 10.1128/jb.129.3.1623-1627.1977. View

16.

Thornsberry C, Jaffee H, Brown S, Edwards T, Biddle J, THOMPSON S . Spectinomycin-resistant Neisseria gonorrhoeae. JAMA. 1977; 237(22):2405-6. View

17.

Dabbs E . A spectinomycin dependent mutant of Escherichia coli. Mol Gen Genet. 1977; 151(3):261-7. DOI: 10.1007/BF00268789. View

18.

Heckels J . The surface of Neisseria gonorrhoeae: isolation of the major components of the outer membrane. J Gen Microbiol. 1977; 99(2):333-41. DOI: 10.1099/00221287-99-2-333. View

19.

Ward M . The bactericidal action of spectinomycin on Neisseria gonorrhoeae. J Antimicrob Chemother. 1977; 3(4):323-9. DOI: 10.1093/jac/3.4.323. View

20.

Swanson J . Surface components affecting interactions between Neisserai gonorrhoeae and eucaryotic cells. J Infect Dis. 1977; 136 Suppl:S138-43. DOI: 10.1093/infdis/136.supplement.s138. View