Xeroderma Pigmentosum Fibroblasts of the D Group Lack an Apurinic DNA Endonuclease Species with a Low Apparent Km

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Mar 1

PMID 643622

Citations 17

Authors

U Kuhnlein

B Lee

E E Penhoet

S Linn

Affiliations

Soon will be listed here.

Abstract

Apurinic DNA endonuclease activity from cultured human fibroblasts was resolved into two species by phosphocellulose chromatography. The species had sedimentation coefficients of 3.3 S and 2.8 S and apparent Km's for apurinic sites of 5 and 44 nM, respectively. The low Km species was absent from extracts of cell lines XP5BE, XP6BE and XP7BE of xeroderma pigmentosum complementation group D.

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