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Purification of Isopenicillin N Synthetase

Overview
Journal Biochem J
Specialty Biochemistry
Date 1984 Sep 15
PMID 6435606
Citations 21
Authors
C P Pang
B Chakravarti
R M Adlington
H H TING
R L White
G S Jayatilake
J E BALDWIN
E P ABRAHAM
Affiliations
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Abstract

Isopenicillin N synthetase was extracted from Cephalosporium acremonium and purified about 200-fold. The product showed one major protein band, coinciding with synthetase activity, when subjected to electrophoresis in polyacrylamide gel. An isopenicillin N synthetase from Penicillium chrysogenum was purified about 70-fold by similar procedures. The two enzymes resemble each other closely in their Mr, in their mobility on electrophoresis in polyacrylamide gel and in their requirement for Fe2+ and ascorbate for maximum activity. Preliminary experiments have shown that a similar isopenicillin N synthetase can be extracted from Streptomyces clavuligerus.

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References
1.
Smith B, Warren S, NEWTON G, ABRAHAM E . Biosynthesis of penicillin N and cephalosporin C. Antibiotic production and other features of the metabolism of Cephalosporium sp. Biochem J. 1967; 103(3):877-90. PMC: 1270493. DOI: 10.1042/bj1030877. View
2.
Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View
3.
LODER P, ABRAHAM E . Isolation and nature of intracellular peptides from a cephalosporin C-producing Cephalosporium sp. Biochem J. 1971; 123(3):471-6. PMC: 1176980. DOI: 10.1042/bj1230471. View
4.
Davies R, ABRAHAM E . Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9. Biochem J. 1974; 143(1):115-27. PMC: 1168359. DOI: 10.1042/bj1430115. View
5.
Fawcett P, Usher J, ABRAHAM E . Behaviour of tritium-labelled isopenicillin N and 6-aminopenicillanic acid as potential penicillin precursors in an extract of Penicillum chrysogenum. Biochem J. 1975; 151(3):741-6. PMC: 1172423. DOI: 10.1042/bj1510741. View