Monoclonal Antibodies to Murine Immunoglobulin Isotypes

Overview

Journal Hybridoma

Publisher Mary Ann Liebert

Specialty Cell Biology

Date 1983 Jan 1

PMID 6432679

Citations 11

Authors

S Weiss

K Lehmann

M Cohn

Affiliations

Soon will be listed here.

Abstract

We have obtained cell lines producing monoclonal antibodies against mu, alpha, lambda, and lambda 1 by fusing the mouse myeloma P3X63Ag8.653 with spleen cells from Lewis rats immunized with several lambda 1 bearing myeloma proteins. The anti-lambda 1 antibody is particularly useful. It was shown to be suitable for radioimmunoassays, for ELISAs when coupled to alkaline phosphatase, and for indirect fluorescence staining of the membrane as well as the cytoplasm.

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Insertion of phosphoglycerine kinase (PGK)-neo 5' of Jlambda1 dramatically enhances VJlambda1 rearrangement.

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Clonal deletion of specific thymocytes by an immunoglobulin idiotype.

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Linkage of the Igl-1 structural and regulatory genes to Akv-2 on chromosome 16.

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Mice completely suppressed for the expression of immunoglobulin kappa light chain.

Weiss S, Lehmann K, Raschke W, Cohn M Proc Natl Acad Sci U S A. 1984; 81(1):211-5.

PMID: 6420788 PMC: 344641. DOI: 10.1073/pnas.81.1.211.