Determination of Mitochondrial Calcium Content in Hepatocytes by a Rapid Cellular-fractionation Technique. Alpha-adrenergic Agonists Do Not Mobilize Mitochondrial Ca2+

Overview

Journal Biochem J

Specialty Biochemistry

Date 1984 Apr 15

PMID 6430266

Citations 8

Authors

S B Shears

C J Kirk

Affiliations

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Abstract

A rapid cellular fractionation technique [the preceding paper, Shears & Kirk (1984) Biochem. J., 219, 375-382] was employed to separate a mitochondria-rich fraction from hepatocytes within seconds. Mitochondrial Ca was estimated to be no more than 41% of total cell Ca. At least half of the mitochondrial Ca was present in an energy-dependent pool; 20% of total cell Ca was accessible to EGTA within 10s. The alpha-adrenergic agonist phenylephrine stimulated glycogen phosphorylase activity by 100% within 0.5 min and induced a loss of 20% of total cell Ca after 10 min from the EGTA-inaccessible pool. However, between 0.5 and 10 min after the addition of phenylephrine to hepatocytes there was no significant change in the Ca content of the mitochondria-rich fraction. Hepatocytes that were preloaded with Ca2+ during 90 min incubation at 0-4 degrees C expelled this cation during 20 min incubation at 37 degrees C. After this time, phenylephrine failed to alter the Ca content of a mitochondria-rich fraction. It is concluded that alpha-adrenergic agonists do not mobilize Ca2+ from hepatocyte mitochondria.

Citing Articles

Determination of mitochondrial calcium content in hepatocytes by a rapid cellular fractionation technique. Vasopressin stimulates mitochondrial Ca2+ uptake.

Shears S, Kirk C Biochem J. 1984; 220(2):417-21.

PMID: 6743279 PMC: 1153642. DOI: 10.1042/bj2200417.

Characterization of a rapid cellular-fractionation technique for hepatocytes. Application in the measurement of mitochondrial membrane potential in situ.

Shears S, Kirk C Biochem J. 1984; 219(2):375-82.

PMID: 6743225 PMC: 1153491. DOI: 10.1042/bj2190375.

The role of calcium ions in the mechanism of action of alpha-adrenergic agonists in rat liver.

Reinhart P, Taylor W, Bygrave F Biochem J. 1984; 223(1):1-13.

PMID: 6149742 PMC: 1144257. DOI: 10.1042/bj2230001.

The Ca2+-mobilizing actions of vasopressin and angiotensin differ from those of the alpha-adrenergic agonist phenylephrine in the perfused rat liver.

Altin J, Bygrave F Biochem J. 1985; 232(3):911-7.

PMID: 4091828 PMC: 1152969. DOI: 10.1042/bj2320911.

Hormone-induced increase in free cytosolic calcium and glycogen phosphorylase activation in rat hepatocytes incubated in normal and low-calcium media.

Binet A, Berthon B, Claret M Biochem J. 1985; 228(3):565-74.

PMID: 4026798 PMC: 1145024. DOI: 10.1042/bj2280565.

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