3-(p-hydroxyphenyl)propionic Acid As a New Fluorogenic Reagent for Amine Oxidase Assays

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1984 Apr 1

PMID 6428269

Citations 8

Authors

T Matsumoto

T Furuta

Y Nimura

O Suzuki

Affiliations

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Abstract

A detailed procedure of a new and extremely sensitive fluorometric assay for amine oxidases is presented. Hydrogen peroxide, produced by the oxidase reaction, reacted with 3-(p-hydroxyphenyl)propionic acid in the presence of peroxidase to yield a fluorescent compound by which enzyme activity could be determined. The enzyme reaction was terminated by NaOH solution, which increased the fluorescence intensity three- to fivefold. The detection limit thus obtained was as little as 0.02 nmol. The alkalinization also contributed to stopping the enzyme reaction and to the clarification of assay mixtures containing turbid enzyme preparations.

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