Microscale Method for Rapid Isolation of Covalently Closed Circular Plasmid DNA from Group N Streptococci

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1984 Apr 1

PMID 6426385

Citations 3

Authors

P K Orberg

W E Sandine

Affiliations

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Abstract

A method for rapid purification of plasmid DNA from lactic streptococci, utilizing microliter quantities of reagents, was developed by combination of a short lysozyme-mutanolysin cell wall digestion with a modification of the Escherichia coli plasmid isolation procedure of McMaster et al. (Anal. Biochem. 109:47-54, 1980). The preparations obtained were highly enriched for covalently closed circular DNA, and the method was applicable to plasmids of at least 40 megadaltons. Centrifugation in CsCl-ethidium bromide density gradients was not required.

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