Translocation of an Immunoglobulin Kappa Locus to a Region 3' of an Unrearranged C-myc Oncogene Enhances C-myc Transcription

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1983 Dec 1

PMID 6424112

Citations 72

Authors

J Erikson

K Nishikura

J Finan

B Emanuel

G Lenoir

P C Nowell

C M Croce

Affiliations

Soon will be listed here.

Abstract

We have studied somatic cell hybrids between mouse myeloma and JI Burkitt lymphoma cells carrying a t(2;8) chromosome translocation for the expression of human kappa chains. and for the presence and rearrangements of the human c-myc oncogene and kappa chain genes. Our results indicate that the c-myc oncogene is unrearranged and remains on the 8q+ chromosome of JI cells. Two rearranged C kappa genes were detected: the expressed allele on normal chromosome 2 and the excluded kappa allele that was translocated from chromosome 2 to the involved chromosome 8 (8q+). The distribution of V kappa and C kappa genes in hybrid clones retaining different human chromosomes indicated that C kappa is distal to V kappa on 2p and that the breakpoint in this Burkitt lymphoma is within the region carrying V kappa genes. High levels of transcripts of the c-myc gene were found when it resided on the 8q+ chromosome but not on the normal chromosome 8, demonstrating that translocation of a kappa locus to region distal to the c-myc oncogene enhances c-myc transcription.

Citing Articles

The FGF/FGFR/c-Myc axis as a promising therapeutic target in multiple myeloma.

Giacomini A, Taranto S, Gazzaroli G, Faletti J, Capoferri D, Marcheselli R J Exp Clin Cancer Res. 2024; 43(1):294.

PMID: 39482742 PMC: 11529022. DOI: 10.1186/s13046-024-03217-2.

Decoding Oncofusions: Unveiling Mechanisms, Clinical Impact, and Prospects for Personalized Cancer Therapies.

Salokas K, Dashi G, Varjosalo M Cancers (Basel). 2023; 15(14).

PMID: 37509339 PMC: 10377698. DOI: 10.3390/cancers15143678.

Medulloblastomics revisited: biological and clinical insights from thousands of patients.

Hovestadt V, Ayrault O, Swartling F, Robinson G, Pfister S, Northcott P Nat Rev Cancer. 2019; 20(1):42-56.

PMID: 31819232 PMC: 9113832. DOI: 10.1038/s41568-019-0223-8.

The PVT1-MYC duet in cancer.

Tseng Y, Bagchi A Mol Cell Oncol. 2016; 2(2):e974467.

PMID: 27308428 PMC: 4904896. DOI: 10.4161/23723556.2014.974467.

A streamlined method for detecting structural variants in cancer genomes by short read paired-end sequencing.

Mijuskovic M, Brown S, Tang Z, Lindsay C, Efstathiadis E, Deriano L PLoS One. 2012; 7(10):e48314.

PMID: 23144753 PMC: 3483208. DOI: 10.1371/journal.pone.0048314.

References

Southern E . Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975; 98(3):503-17. DOI: 10.1016/s0022-2836(75)80083-0. View

Croce C, Shander M, Martinis J, Cicurel L, DAncona G, Dolby T . Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1979; 76(7):3416-9. PMC: 383836. DOI: 10.1073/pnas.76.7.3416. View

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

Sharp P, Berk A, Berget S . Transcription maps of adenovirus. Methods Enzymol. 1980; 65(1):750-68. DOI: 10.1016/s0076-6879(80)65071-x. View

Bentley D, Rabbitts T . Human V kappa immunoglobulin gene number: implications for the origin of antibody diversity. Cell. 1981; 24(3):613-23. DOI: 10.1016/0092-8674(81)90088-x. View

Erikson J, Martinis J, Croce C . Assignment of the genes for human lambda immunoglobulin chains to chromosome 22. Nature. 1981; 294(5837):173-5. DOI: 10.1038/294173a0. View

Watson R, Oskarsson M, Woude G . Human DNA sequence homologous to the transforming gene (mos) of Moloney murine sarcoma virus. Proc Natl Acad Sci U S A. 1982; 79(13):4078-82. PMC: 346580. DOI: 10.1073/pnas.79.13.4078. View

Malcolm S, Barton P, Murphy C, Ferguson-Smith M, Bentley D, Rabbitts T . Localization of human immunoglobulin kappa light chain variable region genes to the short arm of chromosome 2 by in situ hybridization. Proc Natl Acad Sci U S A. 1982; 79(16):4957-61. PMC: 346804. DOI: 10.1073/pnas.79.16.4957. View

Dalla-Favera R, Bregni M, Erikson J, Patterson D, Gallo R, Croce C . Human c-myc onc gene is located on the region of chromosome 8 that is translocated in Burkitt lymphoma cells. Proc Natl Acad Sci U S A. 1982; 79(24):7824-7. PMC: 347441. DOI: 10.1073/pnas.79.24.7824. View

10.

TAUB R, Kirsch I, Morton C, Lenoir G, Swan D, Tronick S . Translocation of the c-myc gene into the immunoglobulin heavy chain locus in human Burkitt lymphoma and murine plasmacytoma cells. Proc Natl Acad Sci U S A. 1982; 79(24):7837-41. PMC: 347444. DOI: 10.1073/pnas.79.24.7837. View

11.

Neel B, Jhanwar S, Chaganti R, Hayward W . Two human c-onc genes are located on the long arm of chromosome 8. Proc Natl Acad Sci U S A. 1982; 79(24):7842-6. PMC: 347445. DOI: 10.1073/pnas.79.24.7842. View

12.

Dalla-Favera R, Martinotti S, Gallo R, Erikson J, Croce C . Translocation and rearrangements of the c-myc oncogene locus in human undifferentiated B-cell lymphomas. Science. 1983; 219(4587):963-7. DOI: 10.1126/science.6401867. View

13.

Erikson J, Drwinga H, Nowell P, Croce C . Transcriptional activation of the translocated c-myc oncogene in burkitt lymphoma. Proc Natl Acad Sci U S A. 1983; 80(3):820-4. PMC: 393472. DOI: 10.1073/pnas.80.3.820. View

14.

Marcu K, Harris L, Stanton L, Erikson J, Watt R, Croce C . Transcriptionally active c-myc oncogene is contained within NIARD, a DNA sequence associated with chromosome translocations in B-cell neoplasia. Proc Natl Acad Sci U S A. 1983; 80(2):519-23. PMC: 393410. DOI: 10.1073/pnas.80.2.519. View

15.

Watt R, Stanton L, Marcu K, Gallo R, Croce C, Rovera G . Nucleotide sequence of cloned cDNA of human c-myc oncogene. Nature. 1983; 303(5919):725-8. DOI: 10.1038/303725a0. View

16.

Gillies S, Morrison S, Oi V, Tonegawa S . A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene. Cell. 1983; 33(3):717-28. DOI: 10.1016/0092-8674(83)90014-4. View

17.

Banerji J, Olson L, Schaffner W . A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain genes. Cell. 1983; 33(3):729-40. DOI: 10.1016/0092-8674(83)90015-6. View

18.

Queen C, Baltimore D . Immunoglobulin gene transcription is activated by downstream sequence elements. Cell. 1983; 33(3):741-8. DOI: 10.1016/0092-8674(83)90016-8. View

19.

Emorine L, Kuehl M, Weir L, Leder P, Max E . A conserved sequence in the immunoglobulin J kappa-C kappa intron: possible enhancer element. Nature. 1983; 304(5925):447-9. DOI: 10.1038/304447a0. View

20.

Nishikura K, Erikson J, Watt R, Rovera G, Croce C . Differential expression of the normal and of the translocated human c-myc oncogenes in B cells. Proc Natl Acad Sci U S A. 1983; 80(15):4822-6. PMC: 384137. DOI: 10.1073/pnas.80.15.4822. View