Monoclonal Antibodies Against Neisseria Meningitidis Lipopolysaccharide

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 Dec 1

PMID 6417025

Citations 9

Authors

R J Sugasawara

C PRATO

J E Sippel

Affiliations

Soon will be listed here.

Abstract

A cell line producing monoclonal antibodies directed against a lipopolysaccharide component of Neisseria meningitidis group A has been established. These antibodies reacted with only one of three lipopolysaccharide serotyping strains of group A meningococci by coagglutination, enzyme-linked immunosorbent assay, and Western blotting techniques. A Western blot analysis showed that a NaOH digest of lipopolysaccharide was detectable by the serotype-specific antibody. The monoclonal antibodies cross-reacted with a group B meningococcal strain in an enzyme-linked immunosorbent assay. The immunoblotting analysis also showed that these antibodies reacted with the lipopolysaccharides of a group B meningococcus as well as Haemophilus influenzae type B, but not with the lipopolysaccharides of several strains of Salmonella typhi, Escherichia coli, Streptococcus pneumoniae, and Neisseria gonorrhoeae.

Citing Articles

Immunoblotting procedure for the analysis of electrophoretically-fractionated bacterial lipopolysaccharide.

Sturm S, Fortnagel P, Timmis K Arch Microbiol. 1984; 140(2-3):198-201.

PMID: 6084988 DOI: 10.1007/BF00454926.

Immunoresponses to Neisseria meningitidis epitopes: primary versus secondary antiphosphorylcholine responses.

Faro J, Seoane R, Puentes E, Martinez Ubeira F, Regueiro B Infect Immun. 1985; 48(2):428-32.

PMID: 2580791 PMC: 261334. DOI: 10.1128/iai.48.2.428-432.1985.

Electromorphic characterization and description of conserved epitopes of the lipooligosaccharides of group A Neisseria meningitidis.

Kim J, Mandrell R, Hu Z, Westerink M, Poolman J, Griffiss J Infect Immun. 1988; 56(10):2631-8.

PMID: 2458319 PMC: 259622. DOI: 10.1128/iai.56.10.2631-2638.1988.

Immunoresponses to Neisseria meningitidis epitopes: in vivo analysis of immunocompetent cells involved in suppression of secondary response to phosphorylcholine.

Faro J, Seoane R, Lareo I, Eiras A, Schiller M, Regueiro B Med Microbiol Immunol. 1987; 176(6):289-303.

PMID: 2448596 DOI: 10.1007/BF00194889.

Immunoresponses to Neisseria meningitidis epitopes: immunomodulation by meningococcus B acts on more than one meningococcal surface epitope.

Faro J, Seoane R, Lareo I, Eiras A, Couceiro J, Regueiro B Med Microbiol Immunol. 1987; 176(3):131-41.

PMID: 2439885 DOI: 10.1007/BF00193894.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hurwitz J, Weissbach A . The formation of 2-keto-3-deoxyheptonic acid in extracts of Escherichia coli B. II. Enzymic studies. J Biol Chem. 1959; 234(4):710-2. View

Frasch C, Gotschlich E . An outer membrane protein of Neisseria meningitidis group B responsible for serotype specificity. J Exp Med. 1974; 140(1):87-104. PMC: 2139694. DOI: 10.1084/jem.140.1.87. View

Higashi G, Sippel J, Girgis N, Hassan A . Counterimmunoelectrophoresis: an adjunct to bacterial culture in the diagnosis of meningococcal meningitis. Scand J Infect Dis. 1974; 6(3):233-5. DOI: 10.3109/inf.1974.6.issue-3.04. View

Sippel J, Quan A . Homogeneity of protein serotype antigens in Neisseria meningitidis group A. Infect Immun. 1977; 16(2):623-7. PMC: 421002. DOI: 10.1128/iai.16.2.623-627.1977. View

Zollinger W, Mandrell R . Outer-membrane protein and lipopolysaccharide serotyping of Neisseria meningitidis by inhibition of a solid-phase radioimmunoassay. Infect Immun. 1977; 18(2):424-33. PMC: 421250. DOI: 10.1128/iai.18.2.424-433.1977. View

James J, Swanson J . Studies on gonococcus infection. XIII. Occurrence of color/opacity colonial variants in clinical cultures. Infect Immun. 1978; 19(1):332-40. PMC: 414084. DOI: 10.1128/iai.19.1.332-340.1978. View

Iscove N, Melchers F . Complete replacement of serum by albumin, transferrin, and soybean lipid in cultures of lipopolysaccharide-reactive B lymphocytes. J Exp Med. 1978; 147(3):923-33. PMC: 2184195. DOI: 10.1084/jem.147.3.923. View

Sippel J, Girgis N . Meningococcal infection in Egypt: laboratory findings in meningitis patients and the prevalence of pharyngeal infection in patients and contacts. Am J Trop Med Hyg. 1978; 27(5):980-5. DOI: 10.4269/ajtmh.1978.27.980. View

10.

Olcen P . Serological methods for rapid diagnosis of haemophilus influenzae, Neisseria meningitidis and Streptococcus pneumoniae in cerebrospinal fluid: a comparison of Co-agglutination, immunofluorescence and immunoelectroosmophoresis. Scand J Infect Dis. 1978; 10(4):283-9. DOI: 10.3109/inf.1978.10.issue-4.05. View

11.

Kennett R . Cell fusion. Methods Enzymol. 1979; 58:345-59. DOI: 10.1016/s0076-6879(79)58149-x. View

12.

Apicella M . Lipopolysaccharide-derived serotype polysaccharides from Neisseria meningitidis group B. J Infect Dis. 1979; 140(1):62-72. DOI: 10.1093/infdis/140.1.62. View

13.

Towbin H, Staehelin T, Gordon J . Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci U S A. 1979; 76(9):4350-4. PMC: 411572. DOI: 10.1073/pnas.76.9.4350. View

14.

Edwards E, Coonrod J . Coagglutination and counterimmunoelectrophoresis for detection of pneumococcal antigens in the sputum of pneumonia patients. J Clin Microbiol. 1980; 11(5):488-91. PMC: 273438. DOI: 10.1128/jcm.11.5.488-491.1980. View

15.

Zollinger W, Mandrell R . Type-specific antigens of group A Neisseria meningitidis: lipopolysaccharide and heat-modifiable outer membrane proteins. Infect Immun. 1980; 28(2):451-8. PMC: 550956. DOI: 10.1128/iai.28.2.451-458.1980. View

16.

de StGroth S, Scheidegger D . Production of monoclonal antibodies: strategy and tactics. J Immunol Methods. 1980; 35(1-2):1-21. DOI: 10.1016/0022-1759(80)90146-5. View

17.

Sippel J, Voller A . Detection of Neisseria meningitidis cell envelope antigen by enzyme-linked immunosorbent assay in patients with meningococcal disease. Trans R Soc Trop Med Hyg. 1980; 74(5):644-8. DOI: 10.1016/0035-9203(80)90156-x. View

18.

Nachamkin I, Cannon J, Mittler R . Monoclonal antibodies against Neisseria gonorrhoeae: production of antibodies directed against a strain-specific cell surface antigen. Infect Immun. 1981; 32(2):641-8. PMC: 351495. DOI: 10.1128/iai.32.2.641-648.1981. View

19.

Burnette W . "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal Biochem. 1981; 112(2):195-203. DOI: 10.1016/0003-2697(81)90281-5. View

20.

Apicella M, Bennett K, Hermerath C, Roberts D . Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 1981; 34(3):751-6. PMC: 350935. DOI: 10.1128/iai.34.3.751-756.1981. View