Sequence-specific DNA Modification in Neisseria Gonorrhoeae

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Sep 1

PMID 6411687

Citations 31

Authors

C Korch

P Hagblom

S Normark

Affiliations

Soon will be listed here.

Abstract

Neisseria gonorrhoeae 82409/55(pJD1) is postulated to possess six DNA sequence-specific cytosine methyltransferases and one DNA sequence-specific N6-adenine methyltransferase. From the DNA sequencing of the plasmid pJD1 (manuscript in preparation) by a modification of the Maxam and Gilbert chemical cleavage procedure, the cytosine methylation specificities were demonstrated. Five of these methylating enzymes and their respective specificities are M . NgoI (formula; see text) does not methylate the cytosine of its recognition sequence, in agreement with a detected adenine modification. A biological implication of these different DNA methylating activities is discussed.

Citing Articles

Identification of type II restriction and modification systems in Helicobacter pylori reveals their substantial diversity among strains.

Xu Q, Morgan R, Roberts R, Blaser M Proc Natl Acad Sci U S A. 2000; 97(17):9671-6.

PMID: 10944229 PMC: 16923. DOI: 10.1073/pnas.97.17.9671.

The Neisseria gonorrhoeae S.NgoVIII restriction/modification system: a type IIs system homologous to the Haemophilus parahaemolyticus HphI restriction/modification system.

Gunn J, Stein D Nucleic Acids Res. 1997; 25(20):4147-52.

PMID: 9321671 PMC: 147021. DOI: 10.1093/nar/25.20.4147.

Use of a non-selective transformation technique to construct a multiply restriction/modification-deficient mutant of Neisseria gonorrhoeae.

Gunn J, Stein D Mol Gen Genet. 1996; 251(5):509-17.

PMID: 8709956 DOI: 10.1007/BF02173639.

Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

Nelson M, Raschke E, McClelland M Nucleic Acids Res. 1993; 21(13):3139-54.

PMID: 8392715 PMC: 309743. DOI: 10.1093/nar/21.13.3139.

Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases.

McClelland M, Nelson M, Raschke E Nucleic Acids Res. 1994; 22(17):3640-59.

PMID: 7937074 PMC: 308336. DOI: 10.1093/nar/22.17.3640.

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