Studies on Endoreduplication. III. Endoreduplication Induced by Mitomycin C in PHA-stimulated Tonsillar Lymphocyte Cultures

Overview

Journal Mutat Res

Publisher Elsevier

Specialty Genetics

Date 1983 Feb 1

PMID 6408469

Citations 3

Authors

H Takanari

K Izutsu

Affiliations

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Abstract

The activity of mitomycin C (MMC) to induce endoreduplication was examined in PHA-stimulated tonsillar lymphocyte cultures. Cellular kinetics of both diploid and endoreduplicated cells was studied by the combined techniques of thymidine and BrdU incorporation. The kinetic data showed that the increase of endoreduplicated cells induced by MMC correlated with the increase of the second-generation diploid cells. Also, it showed that the majority of endoreduplicated cells found at the time when the maximal number of endoreduplicated cells was obtained had incorporated BrdU only once into their diplochromosomes. This suggests that MMC-induced endoreduplicated cells originate from MMC-induced G2-arrested cells.

Citing Articles

Induction of endoreduplication by hydrazine in Chinese hamster V 79 cells and reduced incidence of sister chromatid exchanges in endoreduplicated mitoses.

Speit G, Mehnert K, Vogel W Chromosoma. 1984; 89(1):79-84.

PMID: 6697818 DOI: 10.1007/BF00302355.

Do the frequencies of sister chromatid exchanges in endoreduplicated mitoses provide a measure for lesion persistence and repair?.

Speit G, Vogel W, Mehnert K Chromosoma. 1985; 91(5):369-71.

PMID: 3922698 DOI: 10.1007/BF00291010.

Sensitive period for the induction of endoreduplication by rotenone in cultured Chinese hamster cells.

Matsumoto K, Ohta T Chromosoma. 1992; 102(1):60-5.

PMID: 1291228 DOI: 10.1007/BF00352291.