Genetic Determinant of Pyocin R2 in Pseudomonas Aeruginosa PAO. I. Localization of the Pyocin R2 Gene Cluster Between the TrpCD and TrpE Genes

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1983 Jan 1

PMID 6408350

Citations 18

Authors

T Shinomiya

S Shiga

M Kageyama

Affiliations

Soon will be listed here.

Abstract

Thirty-seven mutants defective in pyocin R2 production in the P. aeruginosa PAO strain were subjected to fine mapping of pyocin R2 genes by transduction with phage F116L. Sixteen complementation groups (designated prtA through prtP) involved in pyocin R2 production were tentatively identified by complementation tests using phage F116L. Their linkages to trpC and trpE markers and fine mapping by three point crosses demonstrated that most of the mutations (prtA through prtN) were located in between trpC and trpE, and that the prtP mutation was localized outside this major prt cluster but in the proximity of the rifA and strA region.

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