Cellular Li+ Opens Paracellular Path in Toad Skin: Amiloride Blockable Effect

Overview

Journal J Membr Biol

Date 1983 Jan 1

PMID 6408262

Citations 6

Authors

J Aboulafia

S M Sanioto

F Lacaz-Vieira

Affiliations

Soon will be listed here.

Abstract

The presence of Li in the solution bathing the outer surface of toad skin under short-circuit condition promotes an unspecific permeability increase characterized by a delayed and progressive increase in the effluxes of 24Na, 42K and 14C sucrose. The effect of Li upon sucrose permeability might indicate an increased permeability of the paracellular pathway. The Li effect is mediated by an intracellular action since blockade of Li entrance into the cell compartment by amiloride prevents the increase in Na, K and sucrose permeability. A possible mechanism of this effect is discussed in terms of a disturbance in the cellular Ca++ balance leading to an increase in cytosolic Ca++ concentration which perturbs the organization of the cytoskeleton and the interplay between cytoskeleton and tight junctions.

Citing Articles

Transient alterations in cellular permeability in cultured human proximal tubule cells: implications for transport studies.

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Deposition of BaSO4 in the tight junctions of amphibian epithelia causes their opening; apical Ca2+ reverses this effect.

Castro J, Sesso A, Lacaz-Vieira F J Membr Biol. 1993; 134(1):15-29.

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Vanadate and ouabain: a comparative study in toad skin.

Aboulafia J, Lacaz-Vieira F Pflugers Arch. 1984; 401(2):204-8.

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Lack of PCMB action upon the outer barrier sodium permeability in the absence of Na in toad skin.

Sanioto S, Aboulafia J Pflugers Arch. 1985; 403(2):115-9.

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Structural and functional response of the isolated toad skin to mucosal lithium.

Sanioto S, Sesso A Pflugers Arch. 1987; 409(1-2):188-93.

PMID: 3039453 DOI: 10.1007/BF00584770.

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