Protein Degradation and Synthesis of Cyanophycin Granule Polypeptide in Aphanocapsa Sp

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1983 Jun 1

PMID 6406434

Citations 4

Authors

M M Allen

M A Hawley

Affiliations

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Abstract

Cyanophycin granule polypeptide content increased by 2- to 3-fold, soluble protein content decreased by 1.5-fold, and carbohydrate content increased by 2-fold within 6 h of chloramphenicol addition to exponentially growing cells of Aphanocapsa sp. strain 6308. Analysis of 14C- and 3H-labeled cells transferred to unlabeled medium and analysis of pulse-labeled cells both suggested cyanophycin granule synthesis from preformed protein breakdown.

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PMID: 24516596 PMC: 3917874. DOI: 10.1371/journal.pone.0088142.

Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and role of three other coisolates in a mixed bacterial consortium.

Sallam A, Steinbuchel A Appl Environ Microbiol. 2008; 74(11):3434-43.

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Engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli.

Hai T, Frey K, Steinbuchel A Appl Environ Microbiol. 2006; 72(12):7652-60.

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Variations in the amino acid composition of cyanophycin in the cyanobacterium Synechocystis sp. PCC 6308 as a function of growth conditions.

Merritt M, Sid S, Mesh L, Allen M Arch Microbiol. 1994; 162(3):158-66.

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References

Allen M, Hutchison F, Weathers P . Cyanophycin granule polypeptide formation and degradation in the cyanobacterium Aphanocapsa 6308. J Bacteriol. 1980; 141(2):687-93. PMC: 293676. DOI: 10.1128/jb.141.2.687-693.1980. View

Allen M, Weathers P . Structure and composition of cyanophycin granules in the cyanobacterium Aphanocapsa 6308. J Bacteriol. 1980; 141(2):959-62. PMC: 293718. DOI: 10.1128/jb.141.2.959-962.1980. View

Simon R . The effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue green alga Anabaena cylindrica. Arch Mikrobiol. 1973; 92(2):115-22. DOI: 10.1007/BF00425009. View

Weathers P, Allen M . Variations in short term products of inorganic carbon fixation in exponential and stationary phase cultures of Aphanocapsa 6308. Arch Microbiol. 1978; 116(3):231-4. DOI: 10.1007/BF00417844. View

Spector T . Refinement of the coomassie blue method of protein quantitation. A simple and linear spectrophotometric assay for less than or equal to 0.5 to 50 microgram of protein. Anal Biochem. 1978; 86(1):142-6. DOI: 10.1016/0003-2697(78)90327-5. View