Potentiation of Lymphocyte Natural Killing by Mixtures of Alpha or Beta Interferon with Recombinant Gamma Interferon

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 Apr 1

PMID 6403469

Citations 16

Authors

D A Weigent

M P Langford

W R Fleischmann Jr

G J STANTON

Affiliations

Soon will be listed here.

Abstract

Human lymphocytes were treated with human alpha (IFN-alpha), beta (IFN-beta), or recombinant gamma (IFN-gamma) interferons separately or in combination to determine their ability to enhance natural killing against mouse L cell targets. Our results showed that recombinant IFN-gamma was approximately 50 times more active per unit of antiviral activity than either IFN-alpha or IFN-beta. Moreover, the levels of natural killing by lymphocytes treated with combinations of IFN-alpha and IFN-beta were additive, whereas combinations of recombinant IFN-gamma and IFN-alpha or recombinant IFN-gamma and IFN-beta were synergistic. The development of natural killing in lymphocytes treated with recombinant IFN-gamma did not occur more rapidly but reached higher levels (62%) than that observed with lymphocytes treated with IFN-alpha or IFN-beta (15%). The results suggest the importance of IFN-gamma and mixtures of IFN-gamma with IFN-alpha or IFN-beta in the enhancement of natural killing activity against virus infections and neoplasia.

Citing Articles

Therapeutic Potential of Interferon-γ and Its Antagonists in Autoinflammation: Lessons from Murine Models of Systemic Juvenile Idiopathic Arthritis and Macrophage Activation Syndrome.

Avau A, Matthys P Pharmaceuticals (Basel). 2015; 8(4):793-815.

PMID: 26610523 PMC: 4695810. DOI: 10.3390/ph8040793.

Natural killer cells utilize both perforin and gamma interferon to regulate murine cytomegalovirus infection in the spleen and liver.

Loh J, Chu D, OGuin A, Yokoyama W, Virgin 4th H J Virol. 2004; 79(1):661-7.

PMID: 15596864 PMC: 538682. DOI: 10.1128/JVI.79.1.661-667.2005.

Inhibition of B16 melanoma experimental metastasis by interferon-gamma through direct inhibition of cell proliferation and activation of antitumour host mechanisms.

Kakuta S, Tagawa Y, Shibata S, Nanno M, Iwakura Y Immunology. 2002; 105(1):92-100.

PMID: 11849319 PMC: 1782640. DOI: 10.1046/j.0019-2805.2001.01342.x.

Interleukin 2 enhances natural killer cell activity through induction of gamma interferon.

Weigent D, STANTON G, Johnson H Infect Immun. 1983; 41(3):992-7.

PMID: 6411624 PMC: 264599. DOI: 10.1128/iai.41.3.992-997.1983.

Alpha interferon: a look to the future.

Bonnem E Invest New Drugs. 1987; 5 Suppl:S65-75.

PMID: 3298135 DOI: 10.1007/BF00207265.

References

Johnson H, Langford M, Lakhchaura B, Chan T, STANTON G . Neutralization of native human gamma interferon (HuIFN gamma) by antibodies to a synthetic peptide encoded by the 5' end of HuIFN gamma cDNA. J Immunol. 1982; 129(6):2357-9. View

Boyum A . Isolation of leucocytes from human blood. Further observations. Methylcellulose, dextran, and ficoll as erythrocyteaggregating agents. Scand J Clin Lab Invest Suppl. 1968; 97:31-50. View

Frayssinet C, GRESSER I, Tovey M, Lindahl P . Letter: Inhibitory effect of potent interferon preparations on the regeneration of mouse liver after partial hepatectomy. Nature. 1973; 245(5421):146-7. DOI: 10.1038/245146b0. View

Gidlund M, Orn A, Wigzell H, Senik A, GRESSER I . Enhanced NK cell activity in mice injected with interferon and interferon inducers. Nature. 1978; 273(5665):759-61. DOI: 10.1038/273759a0. View

Zarling J, Sosman J, Eskra L, Borden E, Horoszewicz J, CARTER W . Enhancement of T cell cytotoxic responses by purified human fibroblast interferon. J Immunol. 1978; 121(5):2002-4. View

Blalock J, Langford M, GEORGIADES J, STANTON G . Nonsensitized lymphocytes produce leukocyte interferon when cultured with foreign cells. Cell Immunol. 1979; 43(1):197-201. DOI: 10.1016/0008-8749(79)90163-1. View

Fleischmann Jr W, Georgiades J, Osborne L, Johnson H . Potentiation of interferon activity by mixed preparations of fibroblast and immune interferon. Infect Immun. 1979; 26(1):248-53. PMC: 414603. DOI: 10.1128/iai.26.1.248-253.1979. View

Langford M, Georgiades J, STANTON G, Dianzani F, Johnson H . Large-scale production and physicochemical characterization of human immune interferon. Infect Immun. 1979; 26(1):36-41. PMC: 414571. DOI: 10.1128/iai.26.1.36-41.1979. View

Fleischmann Jr W, Kleyn K, Baron S . Potentiation of antitumor effect of virus-induced interferon by mouse immune interferon preparations. J Natl Cancer Inst. 1980; 65(5):963-6. View

10.

Langford M, Weigent D, Georgiades J, Johnson H, STANTON G . Antibody to staphylococcal enterotoxin A-induced human immune interferon (IFN gamma). J Immunol. 1981; 126(4):1620-3. View

11.

Kuribayashi K, Gillis S, Kern D, Henney C . Murine NK cell cultures: effects of interleukin-2 and interferon on cell growth and cytotoxic reactivity. J Immunol. 1981; 126(6):2321-7. View

12.

Perussia B, Santoli D, Trinchieri G . Interferon modulation of natural killer cell activity. Ann N Y Acad Sci. 1980; 350:55-62. DOI: 10.1111/j.1749-6632.1980.tb20607.x. View

13.

Brysk M, Tschen E, Hudson R, Smith E, Fleischmann Jr W, Black H . The activity of interferon on ultraviolet light-induced squamous cell carcinomas in mice. J Am Acad Dermatol. 1981; 5(1):61-3. DOI: 10.1016/s0190-9622(81)70078-1. View

14.

Fleischmann Jr W . Potentiation of the direct anticellular activity of mouse interferons: mutual synergism and interferon concentration dependence. Cancer Res. 1982; 42(3):869-75. View

15.

Devos R, Cheroutre H, Taya Y, Degrave W, Van Heuverswyn H, Fiers W . Molecular cloning of human immune interferon cDNA and its expression in eukaryotic cells. Nucleic Acids Res. 1982; 10(8):2487-501. PMC: 320629. DOI: 10.1093/nar/10.8.2487. View