Ultrastructural Aspects of the Effect of Calcium Ionophore A23187 on Incubated Anterior Pituitary Cells of Rats

Overview

Journal Cell Tissue Res

Specialties Anatomy & Histology
Cell Biology

Date 1983 Jan 1

PMID 6403246

Citations 7

Authors

H Fujita

H Kurihara

J Miyagawa

Affiliations

Soon will be listed here.

Abstract

In order to study the fine structural effect of calcium influx on secretory activity of rat anterior pituitary cells, small pieces of anterior pituitary were incubated in Krebs' medium containing the calcium ionophore A23187 (0.15 mM) and were examined electron microscopically. Marked changes were present in all types of secretory cells incubated for 3, 12 and 20 min in the medium containing calcium and A23187. Secretory granules tended to accumulate in the peripheral cytoplasm of the secretory cells, and more numerous images of granule release by exocytosis were observed in somatotroph (STH cell), luteotroph (LTH cell), thyrotroph (TSH cell), corticotroph (ACTH cell), type 1 gonadotroph (Type 1 GTH cell), and type 2 gonadotroph (Type 2 GTH cell). In addition to the increase in the number of exocytosis of single granules, the simultaneous extrusion of multiple granules, "multigranular exocytosis", was often observed in all kinds of secretory cells, especially the ACTH-cells. Large numbers of granule cores were often located in large vacuole-like or channel-like structures, irregular in shape and size, which were open to the intercellular or pericapillary space. Some parts of the membrane of the vacuole-like or channel-like structures were coated. These observations are interpreted to suggest that the calcium influx stimulates the extrusion of the secretory granules by single or multigranular exocytosis.

Citing Articles

Differential regulation of granule-to-granule and granule-to-plasma membrane fusion during secretion from rat pituitary lactotrophs.

Cochilla A, Angleson J, Betz W J Cell Biol. 2000; 150(4):839-48.

PMID: 10953007 PMC: 2175278. DOI: 10.1083/jcb.150.4.839.

Cytochemical studies on the localization and functional properties of calcium in anterior pituitary cells.

Kurihara H, Fujita H Histochemistry. 1984; 81(1):9-13.

PMID: 6432746 DOI: 10.1007/BF00495394.

Ultrastructural demonstration of exocytosis of neural, neuroendocrine and endocrine secretions with an in vitro tannic acid (TARI-) method.

Buma P, Roubos E, Buijs R Histochemistry. 1984; 80(3):247-56.

PMID: 6373682 DOI: 10.1007/BF00495773.

Multigranular exocytosis induced by phospholipase A2-activators, melittin and mastoparan, in rat anterior pituitary cells.

Kurihara H, Kitajima K, Senda T, Fujita H, Nakajima T Cell Tissue Res. 1986; 243(2):311-6.

PMID: 3948236 DOI: 10.1007/BF00251045.

Structural changes at pure cholinergic synaptosomes during the transmitter release induced by A-23187 in Torpedo marmorata. A freeze-fracture study.

Egea G, Esquerda J, Calvet R, Solsona C, Marsal J Cell Tissue Res. 1987; 248(1):207-14.

PMID: 3105889 DOI: 10.1007/BF01239982.

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