Effect of Human Lymphoblastoid Interferon on Insulin Synthesis and Secretion in Isolated Human Pancreatic Islets

Overview

Journal Diabetologia

Specialty Endocrinology

Date 1984 Dec 1

PMID 6397387

Citations 6

Authors

C J Rhodes

K W Taylor

Affiliations

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Abstract

Human islets of Langerhans were isolated from the pancreas removed from a 13-year-old female transplant donor. The islets were incubated in a culture medium for 24 h in the presence of human lymphoblastoid interferon (1000 units/ml). Insulin secretion, proinsulin biosynthesis, total protein biosynthesis and total insulin content were assessed at various concentrations of glucose in the presence of interferon. In interferon-treated islets glucose-stimulated insulin secretion was unaltered from that of control islets; however, glucose-stimulated proinsulin biosynthesis was specifically inhibited by interferon (48%, p less than 0.025). Total protein biosynthesis and total insulin content were not significantly affected by interferon.

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