Isolation of Pigmented and Nonpigmented Mutants of Serratia Marcescens with Reduced Cell Surface Hydrophobicity

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1984 Oct 1

PMID 6384200

Citations 10

Authors

M Rosenberg

Affiliations

Soon will be listed here.

Abstract

Enrichment for nonhydrophobic mutants of Serratia marcescens yielded two types: (i) a nonpigmented mutant which exhibited partial hydrophobic characteristics compared with the wild type, as determined by adherence to hexadecane and polystyrene; and (ii) a pigmented, nonhydrophobic mutant whose colonies were translucent with respect to those of the wild type. The data suggest that the pronounced cell surface hydrophobicity of the wild type is mediated by a combination of several surface factors.

Citing Articles

Filament Negative Regulator Suppresses Glycogen Phosphorylase Encoded That Impacts the Cell Wall-Associated Features in .

Lai W, Hsu H, Cheng C, Wang S, Li W, Hsieh P J Fungi (Basel). 2022; 8(3).

PMID: 35330235 PMC: 8949380. DOI: 10.3390/jof8030233.

Contact-Dependent Growth Inhibition Plays Dual Role in Host-Pathogen Interactions.

Melvin J, Gaston J, Phillips S, Springer M, Marshall C, Shanks R mSphere. 2017; 2(6).

PMID: 29152577 PMC: 5687917. DOI: 10.1128/mSphere.00336-17.

Culturable Aerobic and Facultative Anaerobic Intestinal Bacterial Flora of Black Cobra (Naja naja karachiensis) in Southern Pakistan.

Iqbal J, Sagheer M, Tabassum N, Siddiqui R, Khan N ISRN Vet Sci. 2014; 2014:878479.

PMID: 25002979 PMC: 4060553. DOI: 10.1155/2014/878479.

Serratia marcescens quinoprotein glucose dehydrogenase activity mediates medium acidification and inhibition of prodigiosin production by glucose.

Fender J, Bender C, Stella N, Lahr R, Kalivoda E, Shanks R Appl Environ Microbiol. 2012; 78(17):6225-35.

PMID: 22752173 PMC: 3416624. DOI: 10.1128/AEM.01778-12.

Growth and survival of Serratia marcescens under aerobic and anaerobic conditions in the presence of materials from blood bags.

Szewzyk U, Szewzyk R, Stenstrom T J Clin Microbiol. 1993; 31(7):1826-30.

PMID: 8349760 PMC: 265640. DOI: 10.1128/jcm.31.7.1826-1830.1993.

References

Ding M, Williams R . Biosynthesis of prodigiosin by white strains of Serratia marcescens isolated from patients. J Clin Microbiol. 1983; 17(3):476-80. PMC: 272669. DOI: 10.1128/jcm.17.3.476-480.1983. View

VAN Oss C . Phagocytosis as a surface phenomenon. Annu Rev Microbiol. 1978; 32:19-39. DOI: 10.1146/annurev.mi.32.100178.000315. View

Rosenberg M, Judes H, Weiss E . Cell surface hydrophobicity of dental plaque microorganisms in situ. Infect Immun. 1983; 42(2):831-4. PMC: 264505. DOI: 10.1128/iai.42.2.831-834.1983. View

DISCHE Z . New color reactions for determination of sugars in polysaccharides. Methods Biochem Anal. 1955; 2:313-58. DOI: 10.1002/9780470110188.ch11. View

Rubinovitz C, Gutnick D, Rosenberg E . Emulsan production by Acinetobacter calcoaceticus in the presence of chloramphenicol. J Bacteriol. 1982; 152(1):126-32. PMC: 221383. DOI: 10.1128/jb.152.1.126-132.1982. View

Fattom A, Shilo M . Hydrophobicity as an adhesion mechanism of benthic cyanobacteria. Appl Environ Microbiol. 1984; 47(1):135-43. PMC: 239625. DOI: 10.1128/aem.47.1.135-143.1984. View

Marshall K, Cruickshank R . Cell surface hydrophobicity and the orientation of certain bacteria at interfaces. Arch Mikrobiol. 1973; 91(1):29-40. DOI: 10.1007/BF00409536. View

Cover W, RITTENBERG S . Change in the surface hydrophobicity of substrate cells during bdelloplast formation by Bdellovibrio bacteriovorus 109J. J Bacteriol. 1984; 157(2):391-7. PMC: 215260. DOI: 10.1128/jb.157.2.391-397.1984. View

Blanchard D, Syzdek L . Water-to-Air Transfer and Enrichment of Bacteria in Drops from Bursting Bubbles. Appl Environ Microbiol. 1982; 43(5):1001-5. PMC: 244176. DOI: 10.1128/aem.43.5.1001-1005.1982. View

10.

Purkayastha M, Williams R . Association of pigment with the cell envelope of Serratia marcescens (Chromobacterium prodigiosum). Nature. 1960; 187:349-50. DOI: 10.1038/187349a0. View

11.

Williams R . Biosynthesis of prodigiosin, a secondary metabolite of Serratia marcescens. Appl Microbiol. 1973; 25(3):396-402. PMC: 380817. DOI: 10.1128/am.25.3.396-402.1973. View

12.

Shoham Y, Rosenberg M, Rosenberg E . Bacterial degradation of emulsan. Appl Environ Microbiol. 1983; 46(3):573-9. PMC: 239318. DOI: 10.1128/aem.46.3.573-579.1983. View

13.

Rosenberg E, Gottlieb A, Rosenberg M . Inhibition of bacterial adherence to hydrocarbons and epithelial cells by emulsan. Infect Immun. 1983; 39(3):1024-8. PMC: 348059. DOI: 10.1128/iai.39.3.1024-1028.1983. View

14.

Rosenberg M . Bacterial adherence to polystyrene: a replica method of screening for bacterial hydrophobicity. Appl Environ Microbiol. 1981; 42(2):375-7. PMC: 244016. DOI: 10.1128/aem.42.2.375-377.1981. View

15.

Rosenberg M, Rosenberg E . Role of adherence in growth of Acinetobacter calcoaceticus RAG-1 on hexadecane. J Bacteriol. 1981; 148(1):51-7. PMC: 216165. DOI: 10.1128/jb.148.1.51-57.1981. View