Excretion of Unassembled Flagellin by Salmonella Typhimurium Mutants Deficient in Hook-associated Proteins

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1984 Sep 1

PMID 6384179

Citations 56

Authors

M Homma

H Fujita

S Yamaguchi

T Iino

Affiliations

Soon will be listed here.

Abstract

Of the flagellar filamentless mutants of Salmonella typhimurium, the flaV, flaU, and flaW mutants, which are defective in hook-associated proteins, synthesized flagellin molecules, but flagella did not polymerize at the tips of the mutant hooks and were excreted into the culture medium as intact monomers.

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References

ABRAM D, KOFFLER H, VATTER A . Basal structure and attachment of flagella in cells of Proteus vulgaris. J Bacteriol. 1965; 90(5):1337-54. PMC: 315823. DOI: 10.1128/jb.90.5.1337-1354.1965. View

ABRAM D, VATTER A, KOFFLER H . Attachment and structural features of flagella of certain bacilli. J Bacteriol. 1966; 91(5):2045-68. PMC: 316163. DOI: 10.1128/jb.91.5.2045-2068.1966. View

Hoeniger J, van Iterson W, Van Zanten E . Basal bodies of bacterial flagella in Proteus mirabilis. II. Electron microscopy of negatively stained material. J Cell Biol. 1966; 31(3):603-18. PMC: 2107066. DOI: 10.1083/jcb.31.3.603. View

COHEN-BAZIRE G, London J . Basal organelles of bacterial flagella. J Bacteriol. 1967; 94(2):458-65. PMC: 315060. DOI: 10.1128/jb.94.2.458-465.1967. View

Iino T . Polarity of flagellar growth in salmonella. J Gen Microbiol. 1969; 56(2):227-39. DOI: 10.1099/00221287-56-2-227. View

Holt S, Canale-Parola E . Fine structure of Spirochaeta stenostrepta, a free-living, anaerobic spirochete. J Bacteriol. 1968; 96(3):822-35. PMC: 252378. DOI: 10.1128/jb.96.3.822-835.1968. View

Emerson S, Tokuyasu K, Simon M . Bacterial flagella: polarity of elongation. Science. 1970; 169(3941):190-2. DOI: 10.1126/science.169.3941.190. View

DePamphilis M, Adler J . Fine structure and isolation of the hook-basal body complex of flagella from Escherichia coli and Bacillus subtilis. J Bacteriol. 1971; 105(1):384-95. PMC: 248366. DOI: 10.1128/jb.105.1.384-395.1971. View

DePamphilis M, Adler J . Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane. J Bacteriol. 1971; 105(1):396-407. PMC: 248367. DOI: 10.1128/jb.105.1.396-407.1971. View

10.

OBrien E, Bennett P . Structure of straight flagella from a mutant Salmonella. J Mol Biol. 1972; 70(1):133-52. DOI: 10.1016/0022-2836(72)90168-4. View

11.

Suzuki H, Iino T . In vitro synthesis of phase-specific flagellin of Salmonella. J Mol Biol. 1973; 81(1):57-70. DOI: 10.1016/0022-2836(73)90247-7. View

12.

Iino T . Assembly of Salmonella flagellin in vitro and in vivo. J Supramol Struct. 1974; 2(2-4):372-84. DOI: 10.1002/jss.400020226. View

13.

Suzuki H, Iino T . Absence of messenger ribonucleic acid specific for flagellin in non-flagellate mutants of Salmonella. J Mol Biol. 1975; 95(4):549-56. DOI: 10.1016/0022-2836(75)90316-2. View

14.

Silverman M, Simon M . Bacterial flagella. Annu Rev Microbiol. 1977; 31:397-419. DOI: 10.1146/annurev.mi.31.100177.002145. View

15.

Iino T . Genetics of structure and function of bacterial flagella. Annu Rev Genet. 1977; 11:161-82. DOI: 10.1146/annurev.ge.11.120177.001113. View

16.

Suzuki T, Iino T, Horiguchi T, Yamaguchi S . Incomplete flagellar structures in nonflagellate mutants of Salmonella typhimurium. J Bacteriol. 1978; 133(2):904-15. PMC: 222103. DOI: 10.1128/jb.133.2.904-915.1978. View

17.

Shirakihara Y, Wakabayashi T . Three-dimensional image reconstruction of straight flagella from a mutant Salmonella typhimurium. J Mol Biol. 1979; 131(3):485-507. DOI: 10.1016/0022-2836(79)90004-4. View

18.

Kutsukake K, Iino T, Komeda Y, Yamaguchi S . Functional homology of fla genes between Salmonella typhimurium and Escherichia coli. Mol Gen Genet. 1980; 178(1):59-67. DOI: 10.1007/BF00267213. View

19.

Komeda Y, Kutsukake K, Iino T . Definition of additional flagellar genes in Escherichia coli K12. Genetics. 1980; 94(2):277-90. PMC: 1214143. DOI: 10.1093/genetics/94.2.277. View

20.

Howe J, Hershey J . A sensitive immunoblotting method for measuring protein synthesis initiation factor levels in lysates of Escherichia coli. J Biol Chem. 1981; 256(24):12836-9. View