Heterotopic Tracheal Transplants: Techniques and Applications

Overview

Journal Environ Health Perspect

Date 1984 Jun 1

PMID 6383803

Citations 1

Authors

A J Klein-Szanto

B C Pal

M Terzaghi

A C Marchok

Affiliations

Soon will be listed here.

Abstract

Heterotopic tracheal transplants, placed subcutaneously in syngeneic rats have been extensively used in our laboratory. The objective of these experiments was to study the toxic and/or carcinogenic effect of several compounds on the respiratory tract mucosa. This was attained by exposing the transplants to an intraluminal pettet containing the toxicant or carcinogen mixed with an adequate matrix (gelatin, beeswax, stearyl alcohol, silastic, etc.). By varying the concentration of the test chemicals, it is possible to study dose-response relationships, and by changing the pellet matrix, the effects of release rate (dose rate) can be analyzed. Several end points can be studied, such as histological changes in the mucociliary epithelium, changes in mucus secretion, tumor induction and changes in the in vitro behavior of the epithelial cells after in vivo exposure. In addition, by de-epithelializing the tracheal transplants and reseeding them with another cell population, e.g., from previously treated cell cultures or from human specimens and transplanting them subcutaneously in nude mice, completely new vistas on the effect of chemicals can be opened.

Citing Articles

A tobacco-specific N-nitrosamine or cigarette smoke condensate causes neoplastic transformation of xenotransplanted human bronchial epithelial cells.

Klein-Szanto A, Iizasa T, Momiki S, Caamano J, Metcalf R, Welsh J Proc Natl Acad Sci U S A. 1992; 89(15):6693-7.

PMID: 1323115 PMC: 49569. DOI: 10.1073/pnas.89.15.6693.

References

Kobayashi N, KANISAWA M, Okamoto T, Okita M, Katsuki H . Sequential cytologic study of the development of squamous cell carcinoma induced in subcutaneously implanted bronchial autograft of dog. Acta Cytol. 1978; 22(2):99-104. View

Boutwell R . The function and mechanism of promoters of carcinogenesis. CRC Crit Rev Toxicol. 1974; 2(4):419-43. DOI: 10.3109/10408447309025704. View

Smith W . The tissue transplant technic as a means of testing materials for carcinogenic action. Cancer Res. 1949; 9(12):712-23, illust. View

Watt J, Gregory I, Stell P . A simple method for the post-mortem demonstration of squamous metaplasia in the larynx. J Pathol. 1971; 104(2):149-51. DOI: 10.1002/path.1711040210. View

Klein-Szanto A, Nettesheim P, Topping D, Olson A . Quantitative analysis of disturbed cell maturation in dysplastic lesions of the respiratory tract epithelium. Carcinogenesis. 1980; 1(12):1007-16. DOI: 10.1093/carcin/1.12.1007. View

Ebenstein P, Kinder B, Bankole D, Richards F, Armstrong M . Expression of endogenous xenotropic retrovirus by methylcholanthrene-induced squamous cell carcinoma of the mouse respiratory tract. J Exp Med. 1979; 150(6):1567-70. PMC: 2185731. DOI: 10.1084/jem.150.6.1567. View

Griesemer R, Nettesheim P, Martin D, Caton Jr J . Quantitative exposure of grafted rat tracheas to 7, 12-dimethylbenz(a)anthracene. Cancer Res. 1977; 37(5):1266-71. View

Terzaghi M, Klein-Szanto A . Differentiation of normal and cultured preneoplastic tracheal epithelial cells in rats: importance of epithelial mesenchymal interactions. J Natl Cancer Inst. 1980; 65(5):1039-48. View

Nettesheim P, Klein-Szanto A, Marchok A, Steele V, Terzaghi M, Topping D . Studies of neoplastic development in respiratory tract epithelium. Arch Pathol Lab Med. 1981; 105(1):1-10. View

10.

KNUDTSON K . A method of study of squamous metaplasia, atypical change, and early bronchogenic carcinoma of the bronchial mucosa in smokers and nonsmokers. Cancer. 1956; 9(1):84-5. DOI: 10.1002/1097-0142(195601/02)9:1<84::aid-cncr2820090107>3.0.co;2-f. View

11.

Clark J, Marchok A . Characterization of mucin isolated from rat tracheal transplants. Biochim Biophys Acta. 1979; 588(3):357-67. DOI: 10.1016/0304-4165(79)90344-1. View

12.

Topping D, Nettesheim P . Two-stage carcinogenesis studies with asbestos in Fischer 344 rats. J Natl Cancer Inst. 1980; 65(3):627-30. View

13.

Shors E, Fu P, Matsumura K, Cohen A, Benfield J . Sustained release of benzo(a)pyrene from silicone polymer into the tracheobronchial tree of hamsters and dogs. Cancer Res. 1980; 40(7):2288-94. View

14.

Smith W, Yazdi E, Miller L . Carcinogenesis in pulmonary epithelia in mice on different levels of vitamin A. Environ Res. 1972; 5(2):152-63. DOI: 10.1016/0013-9351(72)90030-8. View

15.

Pal B, Topping D, Griesemer R, Nelson F, Nettesheim P . Development of a system for controlled release of benzo(a)pyrene, 7,12-dimethylbenz(a)anthracene, and phorbol ester for tumor induction in heterotopic tracheal grafts. Cancer Res. 1978; 38(5):1376-83. View

16.

Mossman B, Craighead J . Induction of neoplasms in hamster tracheal grafts with 3-methylcholanthrene-coated Lycra fibers. Cancer Res. 1978; 38(11 Pt 1):3717-22. View

17.

Terzaghi M, Nettesheim P . Dynamics of neoplastic development in carcinogen-exposed tracheal mucosa. Cancer Res. 1979; 39(10):4003-10. View

18.

Craighead J, Mossman B . Carcinoma induction by 3-methylcholanthrene in hamster tracheal tissue implanted in syngeneic animals. Prog Exp Tumor Res. 1979; 24:48-60. DOI: 10.1159/000402083. View

19.

Klein-Szanto A, Topping D, Heckman C, Nettesheim P . Ultrastructural characteristics of carcinogen-induced nondysplastic changes in tracheal epithelium. Am J Pathol. 1980; 98(1):61-82. PMC: 1903400. View

20.

Smith L, Smart V, Ansari G . Mutagenic cholesterol preparations. Mutat Res. 1979; 68(1):23-30. DOI: 10.1016/0165-1218(79)90074-0. View