Guanidine Extraction Enhances the Binding of Human Fibrinogen to Group-B Streptococci

Overview

Journal Med Microbiol Immunol

Specialty Allergy & Immunology

Date 1984 Jan 1

PMID 6381975

Citations 5

Authors

G S Chhatwal

C Lammler

H Blobel

Affiliations

Soon will be listed here.

Abstract

Treatment of group-B streptococci with guanidine chloride significantly enhanced their capacity to bind 125I-labelled fibrinogen. The increase in binding activity was almost proportional in the range of 0.05 M to 6 M guanidine chloride. Repeated extractions with guanidine chloride further increased the capacity of B streptococci to bind the labelled fibrinogen. On the other hand, urea, even at concentrations up to 8 M, did not alter the fibrinogen binding. The enhancement was most pronounced with B streptococci of serotypes III and III-R. Cultivation of these streptococci in Baker and Kasper medium, which is known to stimulate formation of microcapsules, reduced 125I-fibrinogen binding. Subsequent treatment with guanidine increased their binding activity, presumably by facilitating the accessibility of the binding sites.

Citing Articles

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Identification and molecular analysis of PcsB, a protein required for cell wall separation of group B streptococcus.

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References

Markwell M, Haas S, Bieber L, Tolbert N . A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples. Anal Biochem. 1978; 87(1):206-10. DOI: 10.1016/0003-2697(78)90586-9. View

Schonbeck C, Bjorck L, Kronvall G . Receptors for fibrinogen and aggregated beta 2-microglobulin detected in strains of group B streptococci. Infect Immun. 1981; 31(3):856-61. PMC: 351397. DOI: 10.1128/iai.31.3.856-861.1981. View

Kronvall G, Williams Jr R . Immunologic "short circuits". Ann Intern Med. 1969; 70(5):1043-5. DOI: 10.7326/0003-4819-70-5-1043. View

Kronvall G, Schonbeck C, Myhre E . Fibrinogen binding structures in beta-hemolytic streptococci group A, C, and G. Comparisons with receptors for IgG and aggregated beta 2-microglobulin. Acta Pathol Microbiol Scand B. 1979; 87(5):303-10. View

Kawahara K, Kirshner A, Tanford C . DISSOCIATION OF HUMAN CO-hemoglobin by urea, guanidine hydrochloride, and other reagents. Biochemistry. 1965; 4(7):1203-13. DOI: 10.1021/bi00883a001. View

SCHAEG W, Bruckler J, Muller I, Blobel H . ["Clumping factor"-reactions using staphylococci after their extraction with guanidiniumchloride (author's transl)]. Zentralbl Bakteriol Orig A. 1977; 237(4):477-82. View

Chhatwal G, Muller H, Blobel H . Characterization of binding of human alpha 2-macroglobulin to group G streptococci. Infect Immun. 1983; 41(3):959-64. PMC: 264594. DOI: 10.1128/iai.41.3.959-964.1983. View

FOX E . M proteins of group A streptococci. Bacteriol Rev. 1974; 38(1):57-86. PMC: 413841. DOI: 10.1128/br.38.1.57-86.1974. View

Baker C, Kasper D . Microcapsule of type III strains of group B Streptococcus: production and morphology. Infect Immun. 1976; 13(1):189-94. PMC: 420594. DOI: 10.1128/iai.13.1.189-194.1976. View

10.

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

11.

Forsgren A, Grubb A . Many bacterial species bind human IgD. J Immunol. 1979; 122(4):1468-72. View

12.

Kantor F . FIBRINOGEN PRECIPITATION BY STREPTOCOCCAL M PROTEIN. I. IDENTITY OF THE REACTANTS, AND STOICHIOMETRY OF THE REACTION. J Exp Med. 1965; 121:849-59. PMC: 2138005. DOI: 10.1084/jem.121.5.849. View

13.

Switalski L, Ljungh A, Ryden C, Rubin K, Hook M, Wadstrom T . Binding of fibronectin to the surface of group A, C, and G streptococci isolated from human infections. Eur J Clin Microbiol. 1982; 1(6):381-7. DOI: 10.1007/BF02019939. View

14.

Kohler W, PROKOP O . Relationship between haptoglobin and Streptococcus pyogenes T4 antigens. Nature. 1978; 271(5643):373. DOI: 10.1038/271373a0. View

15.

Kronvall G, Myhre E, Bjorck L, BERGGARD I . Binding of aggregated human beta2-microglobulin to surface protein structure in group A, C, and G streptococci. Infect Immun. 1978; 22(1):136-42. PMC: 422127. DOI: 10.1128/iai.22.1.136-142.1978. View

16.

Russell H, Facklam R . Guanidine extraction of streptococcal M protein. Infect Immun. 1975; 12(3):679-86. PMC: 415339. DOI: 10.1128/iai.12.3.679-686.1975. View

17.

Whitnack E, Beachey E . Antiopsonic activity of fibrinogen bound to M protein on the surface of group A streptococci. J Clin Invest. 1982; 69(4):1042-5. PMC: 370160. DOI: 10.1172/jci110508. View

18.

Lammler C, Chhatwal G, Blobel H . Binding of human fibrinogen and its polypeptide chains to group B streptococci. Med Microbiol Immunol. 1983; 172(3):149-53. DOI: 10.1007/BF02123799. View

19.

Shigeoka A, Rote N, Santos J, Hill H . Assessment of the virulence factors of group B streptococci: correlation with sialic acid content. J Infect Dis. 1983; 147(5):857-63. DOI: 10.1093/infdis/147.5.857. View

20.

Blackstock R, Hyde R, Kelly F . Inhibition of fibrinogen reaction by polysaccharide of encapsulated Staphylococcus aureus. J Bacteriol. 1968; 96(3):799-803. PMC: 252375. DOI: 10.1128/jb.96.3.799-803.1968. View