Ocular Antigens VIII: Assay of Delayed Hypersensitivity to Corneal Epithelium in the Rabbit

Overview

Journal Br J Ophthalmol

Specialty Ophthalmology

Date 1978 Feb 1

PMID 638100

Citations 3

Authors

C J Brinkman

R M Broekhuyse

Affiliations

Soon will be listed here.

Abstract

Rabbits were sensitised with complete bovine corneal epithelium. The lymphocyte stimulation test was performed with the lymphocytes of these rabbits using the soluble and sonicated insoluble fraction of the corneal epithelium as the antigens. A striking difference existed in the optimal test conditions for these antigen fractions. By comparing the results of the lymphocyte stimulation test with other immunological parameters, namely, skin test reaction, antibody titre, and phytohaemagglutinin stimulation of the lymphocytes, we concluded that both antigen fractions stimulate predominantly the T-lymphocyte system, although boosting augmented the humoral immune response. Stimulation of the cultured lymphocytes by both the separate and mixed antigen fractions is evidence for the existence of crossreacting antigens between the soluble and insoluble epithelial fractions.

Citing Articles

Immune response evoked by antigen injection in the rabbit vitreous combined with immunopotentiation.

Brinkman C, Winkens H, Broekhuyse R Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1981; 217(3):213-23.

PMID: 6914130 DOI: 10.1007/BF00411152.

Experimental uveitis in isolated humoral and cellular immunity.

Brinkman C, Winkens H, Broekhuyse R Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1979; 210(1):19-30.

PMID: 155413 DOI: 10.1007/BF00414786.

Immunological activity to different corneal antigens in patients with corneal diseases.

Brinkman C, Treffers W, Broekhuyse R Br J Ophthalmol. 1979; 63(10):704-9.

PMID: 116673 PMC: 1043599. DOI: 10.1136/bjo.63.10.704.

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