Comparison of a Forward and a Reverse Mutation Assay in Salmonella Typhimurium Measuring L-arabinose Resistance and Histidine Prototrophy

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 Jun 1

PMID 6378623

Citations 3

Authors

M Ruiz-Rubio

C Hera

C Pueyo

Affiliations

Soon will be listed here.

Abstract

A forward and a reverse mutation assay designed to detect environmental mutagens have been compared in Salmonella typhimurium. The forward mutation assay scored resistance to L-arabinose and the reverse assay, reversion of histidine auxotrophy. Eighteen chemicals of different structural groups, all known to be mutagenic in the histidine reverse assay, were applied to strains carrying the genetic markers needed to perform both mutation assays. The mutagenicity of each chemical was determined by both plate and liquid tests. The plate test counted absolute numbers of surviving mutants and the liquid test separately measured survival and frequency of mutants among the survivors. All the chemicals used were found to be mutagenic in both mutation assays. The response of the L-arabinose assay was equal to or larger than the response of the histidine assay in the case of 16 chemicals. The two other compounds, 2-nitrofluorene and sodium azide, were detected more efficiently by the histidine assay. Sodium azide, a non-carcinogenic compound, is a potent mutagen in the histidine assay, but very weak in the L-arabinose assay.

Citing Articles

Forward and reverse mutations in stages of cancer development.

Hu T, Kumar Y, Shazia I, Duan S, Li Y, Chen L Hum Genomics. 2018; 12(1):40.

PMID: 30134973 PMC: 6104001. DOI: 10.1186/s40246-018-0170-6.

A season-dependent variation of genotoxicity of surface water samples from Taihu Lake, Yangzte delta.

Wu J, Shen L, Gao G, Lin G, Ke X, Shen J Environ Monit Assess. 2004; 98(1-3):225-34.

PMID: 15473538 DOI: 10.1023/b:emas.0000038188.16088.f3.

Toxicity of and mutagenesis by chlorate are independent of nitrate reductase activity in Chlamydomonas reinhardtii.

Prieto R, Fernandez E Mol Gen Genet. 1993; 237(3):429-38.

PMID: 8483458 DOI: 10.1007/BF00279448.

References

Ames B, Gurney E, Miller J, Bartsch H . Carcinogens as frameshift mutagens: metabolites and derivatives of 2-acetylaminofluorene and other aromatic amine carcinogens. Proc Natl Acad Sci U S A. 1972; 69(11):3128-32. PMC: 389719. DOI: 10.1073/pnas.69.11.3128. View

Ames B, Lee F, Durston W . An improved bacterial test system for the detection and classification of mutagens and carcinogens. Proc Natl Acad Sci U S A. 1973; 70(3):782-6. PMC: 433358. DOI: 10.1073/pnas.70.3.782. View

Mohn G, Ellenberger J, McGregor D . Development of mutagenicity test using Escherichia coli K-12 as indicator organism. Mutat Res. 1974; 25(2):187-96. DOI: 10.1016/0027-5107(74)90019-0. View

McCann J, Spingarn N, Kobori J, Ames B . Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids. Proc Natl Acad Sci U S A. 1975; 72(3):979-83. PMC: 432447. DOI: 10.1073/pnas.72.3.979. View

McCann J, Choi E, Yamasaki E, Ames B . Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975; 72(12):5135-9. PMC: 388891. DOI: 10.1073/pnas.72.12.5135. View

Ames B, McCann J, Yamasaki E . Methods for detecting carcinogens and mutagens with the Salmonella/mammalian-microsome mutagenicity test. Mutat Res. 1975; 31(6):347-64. DOI: 10.1016/0165-1161(75)90046-1. View

Green M, Muriel W . Mutagen testing using TRP+ reversion in Escherichia coli. Mutat Res. 1976; 38(1):3-32. DOI: 10.1016/0165-1161(76)90076-5. View

McCann J, Ames B . Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals: discussion. Proc Natl Acad Sci U S A. 1976; 73(3):950-4. PMC: 336038. DOI: 10.1073/pnas.73.3.950. View

MacGregor J, SACKS L . The sporulation system of Bacillus subtilis as the basis of a multi-gene mutagen screening test. Mutat Res. 1976; 38(4):271-86. DOI: 10.1016/0165-1161(76)90151-5. View

10.

Hince T, Neale S . Physiological modification of alkylating agent induced mutagenesis. II. Influence of the numbers of chromosome replicating forks and gene copies on the frequency of mutations induced in Escherichia coli. Mutat Res. 1977; 43(1):11-24. DOI: 10.1016/0027-5107(77)90127-0. View

11.

Skopek T, Liber H, Krolewski J, Thilly W . Quantitative forward mutation assay in Salmonella typhimurium using 8-azaguanine resistance as a genetic marker. Proc Natl Acad Sci U S A. 1978; 75(1):410-4. PMC: 411258. DOI: 10.1073/pnas.75.1.410. View

12.

Skopek T, Liber H, Kaden D, Thilly W . Relative sensitivities of forward and reverse mutation assays in Salmonella typhimurium. Proc Natl Acad Sci U S A. 1978; 75(9):4465-9. PMC: 336136. DOI: 10.1073/pnas.75.9.4465. View

13.

Pueyo C, Cerda-Olmedo E . A mutagen assay detecting forward mutations in an arabinose-sensitive strain of Salmonella typhimurium. Mutat Res. 1978; 54(2):121-9. DOI: 10.1016/0165-1161(78)90032-8. View

14.

Pueyo C . Forward mutations to arabinose resistance in Salmonella typhimurium strains: a sensitive assay for mutagenicity testing. Mutat Res. 1978; 54(3):311-21. DOI: 10.1016/0165-1161(78)90021-3. View

15.

Pueyo C, Frezza D, Smith B . Evaluation of three metabolic activation systems by a forward mutation assay in Salmonella. Mutat Res. 1979; 64(3):183-94. DOI: 10.1016/0165-1161(79)90103-1. View

16.

Pueyo C . Natulan induces forward mutations to L-arabinose-resistance in Salmonella typhimurium. Mutat Res. 1979; 67(2):189-92. DOI: 10.1016/0165-1218(79)90131-9. View

17.

Goze A, Devoret R . Repair promoted by plasmid pKM101 is different from SOS repair. Mutat Res. 1979; 61(2):163-79. DOI: 10.1016/0027-5107(79)90123-4. View

18.

DE Flora S, Coppola R, Zanacchi P, Bennicelli C . Reversal of sodium-azide mutagenicity by liver preparations and by gastric juice. Mutat Res. 1979; 61(2):387-92. DOI: 10.1016/0027-5107(79)90142-8. View

19.

Pueyo C, Lopez-Barea J . The L-arabinose-resistance test with Salmonella typhimurium strain SV3 selects forward mutations at several ara genes. Mutat Res. 1979; 64(4):249-58. DOI: 10.1016/0165-1161(79)90094-3. View

20.

Hollstein M, McCann J, Angelosanto F, Nichols W . Short-term tests for carcinogens and mutagens. Mutat Res. 1979; 65(3):133-226. DOI: 10.1016/0165-1110(79)90014-9. View