Alpha 2.0
Login Register
» Articles » PMID: 6376538

Detection of Clostridium Botulinum Type A Toxin by Enzyme-linked Immunosorbent Assay with Antibodies Produced in Immunologically Tolerant Animals

Overview
Journal J Clin Microbiol
Specialty Microbiology
Date 1984 May 1
PMID 6376538
Citations 21
Authors
M Dezfulian
J G Bartlett
Affiliations
Soon will be listed here.
Abstract

Immunological tolerance is a state of unresponsiveness to foreign substances (antigens) which can develop in human and animal species as the result of continued exposure to antigens early in life. We utilized this principle for the preparation of antibodies against Clostridium botulinum type A toxin. By selective suppression of the immunological response of rabbits to unwanted antigens and subsequent immunization with a toxoid, we were able to produce a specific type A antitoxin without the need to purify the toxin. Despite cross-reactivity with C. botulinum type B, our type A antitoxin was otherwise specific since it did not react with culture filtrates of nontoxigenic variants of type B, any other C. botulinum type (C, D, E, F, and G), nor with 18 other Clostridium species, including Clostridium sporogenes. Using this antitoxin, we developed a sensitive enzyme-linked immunosorbent assay for detection of C. botulinum type A toxin.

Citing Articles

Surface Acoustic Wave Immunosensor for Detection of Botulinum Neurotoxin.

Grabka M, Jasek K, Witkiewicz Z Sensors (Basel). 2023; 23(18).

PMID: 37765744 PMC: 10534944. DOI: 10.3390/s23187688.

Rapid identification of Clostridium botulinum colonies by in vitro toxicity and antimicrobial susceptibility testing.

Dezfulian M, Bartlett J World J Microbiol Biotechnol. 2014; 10(1):27-9.

PMID: 24420880 DOI: 10.1007/BF00357557.

A simple procedure for identification ofClostridium botulinum colonies.

Dezfulian M World J Microbiol Biotechnol. 2014; 9(1):125-7.

PMID: 24419857 DOI: 10.1007/BF00656534.

Sensing the deadliest toxin: technologies for botulinum neurotoxin detection.

capek P, Dickerson T Toxins (Basel). 2011; 2(1):24-53.

PMID: 22069545 PMC: 3206617. DOI: 10.3390/toxins2020024.

Universal and specific quantitative detection of botulinum neurotoxin genes.

Hill B, Skerry J, Smith T, Arnon S, Douek D BMC Microbiol. 2010; 10:267.

PMID: 20961439 PMC: 2973968. DOI: 10.1186/1471-2180-10-267.

References
1.
Lee W, Riemann H . Correlation of toxic and non-toxic strains of Clostridium botulinum by DNA composition and homology. J Gen Microbiol. 1970; 60(1):117-23. DOI: 10.1099/00221287-60-1-117. View
2.
BOROFF D . Radioimmunoassay for type A toxin of Clostridium botulinum. Appl Microbiol. 1973; 25(4):545-9. PMC: 380858. DOI: 10.1128/am.25.4.545-549.1973. View
3.
Sakaguchi G, Sakaguchi S, Kozaki S, Sugii S, Oishi I . Cross reaction in reversed passive hemagglutination between Clostridium botulinum type A and B toxins and its avoidance by the sue of anti-toxic component immunoglobulin isolated by affinity chromatography. Jpn J Med Sci Biol. 1974; 27(3):161-72. DOI: 10.7883/yoken1952.27.161. View
4.
Horwitz M, Hatheway C, Dowell V . Laboratory diagnosis of botulism complicated by pyridostigmine treatment of the patient. A method for selectively removing interfering substances from clinical specimens. Am J Clin Pathol. 1976; 66(4):737-42. DOI: 10.1093/ajcp/66.4.737. View
5.
Notermans S, Dufrenne J, Schothorst M . Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A. Jpn J Med Sci Biol. 1978; 31(1):81-5. DOI: 10.7883/yoken1952.31.81. View