Alpha 2.0
Login Register
» Articles » PMID: 6376337

Monoclonal Antibodies to Rat Renal Antigens

Overview
Journal Immunology
Specialty Allergy & Immunology
Date 1984 Jun 1
PMID 6376337
Citations 4
Authors
F Shimizu
M ORIKASA
K Sato
T Oite
Affiliations
Soon will be listed here.
Abstract

We have developed hybridoma cell lines which secrete monoclonal antibodies to some rat renal antigens, namely the brush border of proximal tubular epithelium and the cytoplasm of tubular cells. The immunoglobulin class of the hybridoma was found to be IgG1. Specific antibody activity against either glomerular basement membrane (GBM) and tubular basement membrane (TBM) or Bowman's capsule and a part of TBM was observed, although these hybridoma cell lines have not yet been successfully established. In particular, the hybridoma secreting antibodies to TBM did not remain stable during antibody production, and was lost during the culture and cloning procedures. These monoclonal antibodies should be of value in research on the pathogenesis of human glomerulonephritis.

Citing Articles

Monoclonal autoantibodies in Heymann nephritis.

Kimura S, Sakurai A, Morioka T, Matsui K, Nakamura T, YAKATA M Clin Exp Immunol. 1986; 65(1):28-33.

PMID: 3539425 PMC: 1542265.

Monoclonal antibodies to human glomerular antigens.

Nakamura T, Oite T, Kazama T, Suzuki S, ORIKASA M, Arakawa M Virchows Arch A Pathol Anat Histopathol. 1988; 412(6):573-82.

PMID: 3129868 DOI: 10.1007/BF00844293.

Monoclonal antibodies against membrane proteins of the rat glomerulus. Immunochemical specificity and immunofluorescence distribution of the antigens.

Miettinen A, Dekan G, Farquhar M Am J Pathol. 1990; 137(4):929-44.

PMID: 1699424 PMC: 1877555.

Epitope-specific induction of mesangial lesions with proteinuria by a MoAb against mesangial cell surface antigen.

Kawachi H, ORIKASA M, Matsui K, Iwanaga T, Toyabe S, Oite T Clin Exp Immunol. 1992; 88(3):399-404.

PMID: 1376651 PMC: 1554519. DOI: 10.1111/j.1365-2249.1992.tb06461.x.

References
1.
Glassock R, Edgington T, Watson J, Dixon F . Autologous immune complex nephritis induced with renal tubular antigen. II. The pathogenetic mechanism. J Exp Med. 1968; 127(3):573-88. PMC: 2138453. DOI: 10.1084/jem.127.3.573. View
2.
Dixon F . The pathogenesis of glomerulonephritis. Am J Med. 1968; 44(4):493-8. DOI: 10.1016/0002-9343(68)90050-8. View
3.
Van Damme B, Fleuren G, Bakker W, Vernier R, HOEDEMAEKER P . Experimental glomerulonephritis in the rat induced by antibodies directed against tubular antigens. V. Fixed glomerular antigens in the pathogenesis of heterologous immune complex glomerulonephritis. Lab Invest. 1978; 38(4):502-10. View
4.
Couser W, Steinmuller D, Stilmant M, Salant D, LOWENSTEIN L . Experimental glomerulonephritis in the isolated perfused rat kidney. J Clin Invest. 1978; 62(6):1275-87. PMC: 371893. DOI: 10.1172/JCI109248. View
5.
Sakai L, Engvall E, Hollister D, Burgeson R . Production and characterization of a monoclonal antibody to human Type IV collagen. Am J Pathol. 1982; 108(3):310-8. PMC: 1916037. View