Glial Fibrillary Acidic Protein is Localized in the Lens Epithelium

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1984 May 1

PMID 6373785

Citations 17

Authors

J S Hatfield

R P Skoff

H Maisel

L Eng

Affiliations

Soon will be listed here.

Abstract

The epithelium of the mouse lens stains intensely with antisera to glial fibrillary acidic protein (GFAP). A protein co-migrating with GFAP and immunoreactive with antisera to GFAP can be demonstrated in lens epithelium protein extracts by immunoblots. GFAP has previously been considered unique to cells of neural origin, but this study demonstrates that ectodermally derived cells express GFAP or a highly similar protein.

Citing Articles

A simple, rapid fluorescent reporter-based method for detection of ectopic cre recombinase expression in presumed retinal cell type-targeted mouse lines.

Ramachandra Rao S, Fliesler S Exp Eye Res. 2023; 235:109637.

PMID: 37659708 PMC: 10756212. DOI: 10.1016/j.exer.2023.109637.

Qki activates Srebp2-mediated cholesterol biosynthesis for maintenance of eye lens transparency.

Shin S, Zhou H, He C, Wei Y, Wang Y, Shingu T Nat Commun. 2021; 12(1):3005.

PMID: 34021134 PMC: 8139980. DOI: 10.1038/s41467-021-22782-0.

Surgical stress and cytoskeletal changes in lens epithelial cells following manual and femtosecond laser-assisted capsulotomy.

Sukosd A, Szabadfi K, Szabo-Meleg E, Gaspar B, Stodulka P, Setalo Jr G Int J Ophthalmol. 2020; 13(6):927-934.

PMID: 32566504 PMC: 7270246. DOI: 10.18240/ijo.2020.06.11.

Expression of the type VI intermediate filament proteins CP49 and filensin in the mouse lens epithelium.

FitzGerald P, Sun N, Shibata B, Hess J Mol Vis. 2016; 22:970-89.

PMID: 27559293 PMC: 4975932.

Adenomatous polyposis coli is essential for both neuronal differentiation and maintenance of adult neural stem cells in subventricular zone and hippocampus.

Imura T, Wang X, Noda T, Sofroniew M, Fushiki S Stem Cells. 2010; 28(11):2053-2064.

PMID: 21089118 PMC: 4524517. DOI: 10.1002/stem.524.

References

Amaducci L, Forno K, Eng L . Glial fibrillary acidic protein in cryogenic lesions of the rat brain. Neurosci Lett. 1981; 21(1):27-32. DOI: 10.1016/0304-3940(81)90052-5. View

Yen S, Fields K . Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol. 1981; 88(1):115-26. PMC: 2111704. DOI: 10.1083/jcb.88.1.115. View

Suess U, Pliska V . Identification of the pituicytes as astroglial cells by indirect immunofluorescence-staining for the glial fibrillary acidic protein. Brain Res. 1981; 221(1):27-33. DOI: 10.1016/0006-8993(81)91061-1. View

Dixon R, Eng L . Glial fibrillary acidic protein in the optic nerve of the developing albino rat: an immunoperoxidase study of paraffin-embedded tissue. J Comp Neurol. 1981; 201(1):15-24. DOI: 10.1002/cne.902010103. View

Schnitzer J, Franke W, Schachner M . Immunocytochemical demonstration of vimentin in astrocytes and ependymal cells of developing and adult mouse nervous system. J Cell Biol. 1981; 90(2):435-47. PMC: 2111851. DOI: 10.1083/jcb.90.2.435. View

Salm A, Hatton G, Nilaver G . Immunoreactive glial fibrillary acidic protein in pituicytes of the rat neurohypophysis. Brain Res. 1982; 236(2):471-6. DOI: 10.1016/0006-8993(82)90729-6. View

Trimmer P, Reier P, Oh T, Eng L . An ultrastructural and immunocytochemical study of astrocytic differentiation in vitro: changes in the composition and distribution of the cellular cytoskeleton. J Neuroimmunol. 1982; 2(3-4):235-60. DOI: 10.1016/0165-5728(82)90058-3. View

Nakazato Y, Ishizeki J, Takahashi K, Yamaguchi H, Kamei T, Mori T . Localization of S-100 protein and glial fibrillary acidic protein-related antigen in pleomorphic adenoma of the salivary glands. Lab Invest. 1982; 46(6):621-6. View

Bignami A, Raju T, Dahl D . Localization of vimentin, the nonspecific intermediate filament protein, in embryonal glia and in early differentiating neurons. In vivo and in vitro immunofluorescence study of the rat embryo with vimentin and neurofilament antisera. Dev Biol. 1982; 91(2):286-95. DOI: 10.1016/0012-1606(82)90035-5. View

10.

Levitt P, Cooper M, Rakic P . Coexistence of neuronal and glial precursor cells in the cerebral ventricular zone of the fetal monkey: an ultrastructural immunoperoxidase analysis. J Neurosci. 1981; 1(1):27-39. PMC: 6564162. View

11.

Dahl D, Chi N, Miles L, Nguyen B, Bignami A . Glial fibrillary acidic (GFA) protein in Schwann cells: fact or artifact?. J Histochem Cytochem. 1982; 30(9):912-8. DOI: 10.1177/30.9.6182187. View

12.

Coons A . Fluorescent antibody methods. Gen Cytochem Methods. 1958; 1:399-422. View

13.

ORAHILLY R, Meyer D . The early development of the eye in the chick Gallus domesticus (stages 8 to 25). Acta Anat (Basel). 1959; 36(1-2):20-58. DOI: 10.1159/000141425. View

14.

Nakane P, Pierce Jr G . Enzyme-labeled antibodies: preparation and application for the localization of antigens. J Histochem Cytochem. 1966; 14(12):929-31. DOI: 10.1177/14.12.929. View

15.

STERNBERGER L, HARDY Jr P, CUCULIS J, MEYER H . The unlabeled antibody enzyme method of immunohistochemistry: preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J Histochem Cytochem. 1970; 18(5):315-33. DOI: 10.1177/18.5.315. View

16.

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

17.

Eng L, Vanderhaeghen J, Bignami A, Gerstl B . An acidic protein isolated from fibrous astrocytes. Brain Res. 1971; 28(2):351-4. DOI: 10.1016/0006-8993(71)90668-8. View

18.

UYEDA C, Eng L, Bignami A . Immunological study of the glial fibrillary acidic protein. Brain Res. 1972; 37(1):81-9. DOI: 10.1016/0006-8993(72)90347-2. View

19.

Gabella G . Fine structure of the myenteric plexus in the guinea-pig ileum. J Anat. 1972; 111(Pt 1):69-97. PMC: 1271115. View

20.

Bignami A, Eng L, Dahl D, UYEDA C . Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 1972; 43(2):429-35. DOI: 10.1016/0006-8993(72)90398-8. View