Identification of Thymostimulin Secreting Cells in Calf Thymus by Immunoperoxidase Method

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1984 Jan 1

PMID 6373680

Citations 1

Authors

M Aita

D Cocchia

A B Minella

A Amantea

Affiliations

Soon will be listed here.

Abstract

An anti- thymostimulin (TS) serum was tested on calf thymus to study the localization of the hormonal factor. The immunoperoxidase method was applied to tissue fixed in Bouin's fluid and embedded in paraffin, or to tissue fixed in paraformaldehyde and embedded in Epon for semi-thin sections. Immuno-reactivity was shown, with DAB- Fluka , in reticulo-epithelial cells in the medulla, and between the cortex and the medulla, while with DAB-Sigma reactivity was found in the cortex as well. The external cells of Hassall's corpuscles were also reactive. Myoid cells were not reactive. In semi-thin sections a weak reactivity was noted at the periphery of a few lymphocytes. Comparison with the localization of other thymic factors, and the possibility of a functional cycle of the epithelial cells synthesizing one or more factors are discussed.

Citing Articles

Effects of hypophyseal or thymic allograft on thymus development in partially decerebrate chicken embryos: expression of PCNA and CD3 markers.

Aita M, Benedetti F, Carafelli E, Caccia E, Romano N Eur J Histochem. 2010; 54(3):e37.

PMID: 20819775 PMC: 3167313. DOI: 10.4081/ejh.2010.e37.

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