Detection of Salmonella Spp. in Milk by Using Felix-O1 Bacteriophage and High-pressure Liquid Chromatography

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1983 Nov 1

PMID 6360047

Citations 2

Authors

D C Hirsh

L D Martin

Affiliations

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Abstract

A method is described whereby the presence of less than five salmonellae was detected per milliliter of milk within 24 h of sample collection. Salmonellae were removed from milk by means of electropositive large-pore filters. Eluates from the filters were analyzed for the presence of Salmonella spp. by Felix-O1 bacteriophage and high-pressure liquid chromatographic techniques. The method gave only a positive response when salmonellae were present in the milk. Of the serotypes and strains of Salmonella spp. tested, Salmonella dublin (10 strains), Salmonella typhimurium (5 strains), Salmonella anatum, Salmonella krefeld, and Salmonella saint-paul gave positive responses. One strain of Salmonella agona (three strains tested) and three strains of Salmonella enteritidis (seven strains tested) were not detectable by the method described herein.

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References

Carlson V, SNOEYENBOS G, McKie B, SMYSER C . A comparison of incubation time and temperature for the isolation of salmonella. Avian Dis. 1967; 11(2):217-25. View

Kallings L . Sensitivity of various salmonella strains to felix 0-1 phage. Acta Pathol Microbiol Scand. 1967; 70(3):446-54. DOI: 10.1111/j.1699-0463.1967.tb01312.x. View

Kallings L, Lindberg A . Resistance to Felix 0-1 phage in salmonella bacteria. Acta Pathol Microbiol Scand. 1967; 70(3):455-60. DOI: 10.1111/j.1699-0463.1967.tb01313.x. View

Sperber W, Deibel R . Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969; 17(4):533-9. PMC: 377737. DOI: 10.1128/am.17.4.533-539.1969. View

Lindberg A, Holme T . Influence of O side chains on the attachment of the Felix O-1 bacteriophage to Salmonella bacteria. J Bacteriol. 1969; 99(2):513-9. PMC: 250048. DOI: 10.1128/jb.99.2.513-519.1969. View

Sorrells K, Speck M, Warren J . Pathogenicity of Salmonella gallinarum after metabolic injury by freezing. Appl Microbiol. 1970; 19(1):39-43. PMC: 376605. DOI: 10.1128/am.19.1.39-43.1970. View

Morris G, Dunn C . Influence of incubation temperature and sodium heptadecyl sulfate (Tergitol No. 7) on the isolation of salmonellae from pork sausage. Appl Microbiol. 1970; 20(2):192-5. PMC: 376898. DOI: 10.1128/am.20.2.192-195.1970. View

SMYSER C, SNOEYENBOS G . Enrichment serology compared with a direct-culture procedure for isolating salmonellae from rendered animal by-products. Avian Dis. 1971; 15(3):581-7. View

Welkos S, Schreiber M, Baer H . Identification of Salmonella with the O-1 bacteriophage. Appl Microbiol. 1974; 28(4):618-22. PMC: 186784. DOI: 10.1128/am.28.4.618-622.1974. View

10.

Gunnarsson A, Hurvell B, THAL E . Recent experiences with the Salmonella-O-1-phage in routine diagnostic work. Zentralbl Bakteriol Orig A. 1977; 237(2-3):222-7. View

11.

Krysinski E, Heimsch R . Use of enzyme-labeled antibodies to detect Salmonella in foods. Appl Environ Microbiol. 1977; 33(4):947-54. PMC: 170795. DOI: 10.1128/aem.33.4.947-954.1977. View

12.

Gerba C, Farrah S, Goyal S, Wallis C, Melnick J . Concentration of enteroviruses from large volumes of tap water, treated sewage, and seawater. Appl Environ Microbiol. 1978; 35(3):540-8. PMC: 242876. DOI: 10.1128/aem.35.3.540-548.1978. View

13.

Swaminathan B, Ayres J, Williams J . Control of nonspecific staining in the fluorescent antibody technique for the detection of salmonellae in foods. Appl Environ Microbiol. 1978; 35(5):911-9. PMC: 242952. DOI: 10.1128/aem.35.5.911-919.1978. View

14.

Werner S, HUMPHREY G, Kamei I . Association between raw milk and human Salmonella dublin infection. Br Med J. 1979; 2(6184):238-41. PMC: 1595604. DOI: 10.1136/bmj.2.6184.238. View

15.

Goyal S, Hanssen H, Gerba C . Simple method for the concentration of influenza virus from allantoic fluid on microporous filters. Appl Environ Microbiol. 1980; 39(3):500-4. PMC: 291367. DOI: 10.1128/aem.39.3.500-504.1980. View

16.

Males B, Stocker B . Escherichia coli K317, formerly used to define colicin group E2, produces colicin E7, is immune to colicin E2, and carries a bacteriophage-restricting conjugative plasmid. J Bacteriol. 1980; 144(2):524-31. PMC: 294699. DOI: 10.1128/jb.144.2.524-531.1980. View

17.

Hou K, Gerba C, Goyal S, Zerda K . Capture of latex beads, bacteria, endotoxin, and viruses by charge-modified filters. Appl Environ Microbiol. 1980; 40(5):892-6. PMC: 291685. DOI: 10.1128/aem.40.5.892-896.1980. View

18.

Goyal S, Gerba C . Simple method for concentration of bacteria from large volumes of tap water. Appl Environ Microbiol. 1980; 40(5):912-6. PMC: 291689. DOI: 10.1128/aem.40.5.912-916.1980. View

19.

SANBORN W, Lesmana M, Edwards E . Enrichment culture coagglutination test for rapid, low-cost diagnosis of salmonellosis. J Clin Microbiol. 1980; 12(2):151-5. PMC: 273545. DOI: 10.1128/jcm.12.2.151-155.1980. View

20.

Minnich S, Hartman P, Heimsch R . Enzyme immunoassay for detection of Salmonellae in foods. Appl Environ Microbiol. 1982; 43(4):877-93. PMC: 241935. DOI: 10.1128/aem.43.4.877-893.1982. View