Purification of Rat Liver Enzymes Involved in the Oxidation of Glyoxylate
Overview
Biophysics
Affiliations
The use of Sepharose aminohexyl oxamate for the purification of glycolate oxidase and lactate dehydrogenase is described. The kinetics of both enzymes are reported in relation to their possible roles in the production of oxalate. A model is proposed in which glycolate oxidase in the peroxisomes and lactate dehydrogenase in the cytosol cooperate in the production of oxalate.
Danpure C, Jennings P, Mistry J, Chalmers R, McKerrell R, Blakemore W J Inherit Metab Dis. 1989; 12(4):403-14.
PMID: 2516173 DOI: 10.1007/BF01802035.
Recent advances in the understanding, diagnosis and treatment of primary hyperoxaluria type 1.
Danpure C J Inherit Metab Dis. 1989; 12(2):210-24.
PMID: 2502679 DOI: 10.1007/BF01800727.