Production of Monoclonal Antibodies to Hepatitis B Surface and Core Antigens, and Use in the Detection of Viral Antigens in Liver Biopsies

Overview

Journal J Hyg (Lond)

Publisher Cambridge University Press

Specialty Public Health

Date 1983 Feb 1

PMID 6337208

Citations 9

Authors

R S Tedder

P Guarascio

J L Yao

R B Lord

A L Eddleston

Affiliations

Soon will be listed here.

Abstract

Hybridomas secreting monoclonal antibodies to HBsAg and HBcAg were prepared from immunized mice. An antibody capture radioimmunoassay was used to detect and select appropriate hybrids for propagation and cloning. The advantages of this assay were discussed. The resulting monoclonal antibodies were compared with conventional polyclonal antisera for the detection of virus antigens in liver tissue and found to give excellent results.

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