The Production of Hybridomas from the Gut Associated Lymphoid Tissue of Tumour Bearing Rats. II. Peripheral Intestinal Lymph As a Source of IgA Producing Cells

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1984 Aug 1

PMID 6331925

Citations 6

Authors

J M Styles

C J Dean

L A Gyure

S M Hobbs

J G Hall

Affiliations

Soon will be listed here.

Abstract

Peripheral intestinal lymph afferent to the mesenteric nodes has been collected from rats bearing syngeneic sarcomata in their Peyer's patches and the B cells used to produce rat X rat hybridomas. Analysis of the hybridoma supernatants by radioimmunoassay for the presence of immunoglobulins, showed that hybridomas secreting IgA predominated. Eleven out of the 15 hybridomas selected for antibody binding to cells of the immunising tumour secreted IgA antibodies, and six of these were tumour specific. Efferent mesenteric lymph (i.e. normal thoracic duct lymph), on the other hand, was found to be a poor source of B cells for hybridoma production and no specific IgA secreting hybridomas were obtained. The high yield of IgA secreting hybridomas obtained shows that peripheral intestinal lymph is a better source of IgA committed B cells than are the mesenteric nodes or thoracic duct lymph. We conclude that the IgA producing cells in the latter tissues are too far along the differentiation pathway to plasma cells to undergo successful somatic cell fusion.

Citing Articles

The shedding of viable cells into the local lymph by tumours growing in the gut of rats.

Gyure L, Styles J, Dean C, Nagy K, Hall J Br J Cancer. 1985; 51(3):379-82.

PMID: 3970813 PMC: 1976939. DOI: 10.1038/bjc.1985.50.

Immunity to a syngeneic sarcoma induced in rats by dendritic lymph cells exposed to the tumour either in vivo or in vitro.

Gyure L, Barfoot R, Denham S, Hall J Br J Cancer. 1987; 55(1):17-20.

PMID: 3814471 PMC: 2001578. DOI: 10.1038/bjc.1987.4.

A receptor for monomeric IgG2b on rat macrophages.

Denham S, Barfoot R, Jackson E Immunology. 1987; 62(1):69-74.

PMID: 2958407 PMC: 1453711.

Some properties of dendritic macrophages from peripheral lymph.

Barfoot R, Denham S, Gyure L, Hall J, Hobbs S, Jackson L Immunology. 1989; 68(2):233-9.

PMID: 2807381 PMC: 1385423.

Pharmacokinetic studies of radiolabelled rat monoclonal antibodies recognising syngeneic sarcoma antigens. I. Comparison of IgG subclasses.

Eccles S, Purvies H, Styles J, Hobbs S, Dean C Cancer Immunol Immunother. 1989; 30(1):5-12.

PMID: 2598175 PMC: 11038649. DOI: 10.1007/BF01665024.

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