A Novel Method for Site-directed Mutagenesis: Its Application to an Eukaryotic TRNAPro Gene Promoter

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1982 Jan 1

PMID 6329678

Citations 35

Authors

C Traboni

G Ciliberto

R Cortese

Affiliations

Soon will be listed here.

Abstract

We present a novel general method for localized mutagenesis. The DNA segment to be mutagenized is inserted in the beta-galactosidase gene of a M13-lac vector, generally causing loss of beta-galactosidase function by generation of frameshifts or nonsense codons. Mutations in the inserted DNA which restore beta-galactosidase function are readily detected and analyzed. The application of this method to the promoter of an eukaryotic (Caenorhabditis elegans) tRNAPro gene has allowed the isolation of several mutants altered in transcription.

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