Effect of Different Collagenases on the Isolation of Viable Hepatocytes from Rat Liver

Overview

Journal Anal Biochem

Publisher Elsevier

Specialty Biochemistry

Date 1984 Apr 1

PMID 6329035

Citations 4

Authors

A E Queral

A B DeAngelo

C T Garrett

Affiliations

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Abstract

The isolation of viable hepatocytes from rat liver was found to depend on the source of collagenase (EC 3.4.24.3) more than any other single factor examined. Collagenase purified from Achromobacter iophagus/Bacillus polymyxa (collagenase/dispase) gave reproducibly high viability without the use of complex perfusion protocols.

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