Critical Spatial Requirement Within the Origin of Simian Virus 40 DNA Replication

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 1984 Jul 1

PMID 6328047

Citations 26

Authors

G L Cohen

P J Wright

A L DeLucia

B A Lewton

M E Anderson

P Tegtmeyer

Affiliations

Soon will be listed here.

Abstract

We inserted a single base pair into the center of a 27-base-pair palindrome within the replication origin of simian virus 40. The mutation did not directly alter the symmetry of the palindrome or the protein-binding sequences within the palindrome. DNA binding studies showed that subunits of the simian virus 40 A protein (T antigen) bound to each of the four recognition pentanucleotides in the origin palindrome but did so with reduced affinity in comparison with wild-type origins. The mutant origin cloned in a plasmid DNA failed to replicate in COS cells. Thus, precise spatial interactions among subunits of A protein are necessary for stable origin binding and are crucial for subsequent steps in the initiation of DNA replication. Furthermore, any possible functional interactions of the simian virus 40 A protein with cellular DNA would require a great fidelity of protein binding arrangements to initiate cellular DNA replication.

Citing Articles

ATM and ATR activities maintain replication fork integrity during SV40 chromatin replication.

Sowd G, Li N, Fanning E PLoS Pathog. 2013; 9(4):e1003283.

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Preformed hexamers of SV40 T antigen are active in RNA and origin-DNA unwinding.

Uhlmann-Schiffler H, Seinsoth S, Stahl H Nucleic Acids Res. 2002; 30(14):3192-201.

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The Chinese hamster dihydrofolate reductase replication origin beta is active at multiple ectopic chromosomal locations and requires specific DNA sequence elements for activity.

Altman A, Fanning E Mol Cell Biol. 2001; 21(4):1098-110.

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Replication but not transcription of simian virus 40 DNA is dependent on nuclear domain 10.

Tang Q, Bell P, Tegtmeyer P, Maul G J Virol. 2000; 74(20):9694-700.

PMID: 11000241 PMC: 112401. DOI: 10.1128/jvi.74.20.9694-9700.2000.

The simian virus 40 core origin contains two separate sequence modules that support T-antigen double-hexamer assembly.

Sreekumar K, Prack A, Winters D, Barbaro B, Bullock P J Virol. 2000; 74(18):8589-600.

PMID: 10954561 PMC: 116372. DOI: 10.1128/jvi.74.18.8589-8600.2000.

References

Hirt B . Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967; 26(2):365-9. DOI: 10.1016/0022-2836(67)90307-5. View

Lewton B, DeLucia A, Tegtmeyer P . Binding of simian virus 40 a protein to DNA with deletions at the origin of replication. J Virol. 1984; 49(1):9-13. PMC: 255418. DOI: 10.1128/JVI.49.1.9-13.1984. View

Tegtmeyer P . Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon. J Virol. 1972; 10(4):591-8. PMC: 356508. DOI: 10.1128/JVI.10.4.591-598.1972. View

Danna K, Nathans D . Bidirectional replication of Simian Virus 40 DNA. Proc Natl Acad Sci U S A. 1972; 69(11):3097-100. PMC: 389712. DOI: 10.1073/pnas.69.11.3097. View

Chou J, Avila J, Martin R . Viral DNA synthesis in cells infected by temperature-sensitive mutants of simian virus 40. J Virol. 1974; 14(1):116-24. PMC: 355484. DOI: 10.1128/JVI.14.1.116-124.1974. View

Martin R, Chou J . Simian virus 40 functions required for the establishment and maintenance of malignant transformation. J Virol. 1975; 15(3):599-612. PMC: 354496. DOI: 10.1128/JVI.15.3.599-612.1975. View

Tegtmeyer P . Function of simian virus 40 gene A in transforming infection. J Virol. 1975; 15(3):613-8. PMC: 354497. DOI: 10.1128/JVI.15.3.613-618.1975. View

Brugge J, Butel J . Role of simian virus 40 gene A function in maintenance of transformation. J Virol. 1975; 15(3):619-35. PMC: 354498. DOI: 10.1128/JVI.15.3.619-635.1975. View

Osborn M, Weber K . Simian virus 40 gene A function and maintenance of transformation. J Virol. 1975; 15(3):636-44. PMC: 354499. DOI: 10.1128/JVI.15.3.636-644.1975. View

10.

Martin R, OPPENHEIM A . Initiation points for DNA replication in nontransformed and simian virus 40-transformed Chinese hamster lung cells. Cell. 1977; 11(4):859-69. DOI: 10.1016/0092-8674(77)90297-5. View

11.

Tjian R . The binding site on SV40 DNA for a T antigen-related protein. Cell. 1978; 13(1):165-79. DOI: 10.1016/0092-8674(78)90147-2. View

12.

Tjian R, Fey G, Graessmann A . Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A. 1978; 75(3):1279-83. PMC: 411454. DOI: 10.1073/pnas.75.3.1279. View

13.

Shortle D, Nathans D . Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome. Proc Natl Acad Sci U S A. 1978; 75(5):2170-4. PMC: 392513. DOI: 10.1073/pnas.75.5.2170. View

14.

Subramanian K, Shenk T . Definition of the boundaries of the origin of DNA replication in simian virus 40. Nucleic Acids Res. 1978; 5(10):3635-42. PMC: 342700. DOI: 10.1093/nar/5.10.3635. View

15.

Shortle D, Margolskee R, Nathans D . Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin. Proc Natl Acad Sci U S A. 1979; 76(12):6128-31. PMC: 411816. DOI: 10.1073/pnas.76.12.6128. View

16.

DiMaio D, Nathans D . Cold-sensitive regulatory mutants of simian virus 40. J Mol Biol. 1980; 140(1):129-42. DOI: 10.1016/0022-2836(80)90359-9. View

17.

Gluzman Y, Sambrook J, Frisque R . Expression of early genes of origin-defective mutants of simian virus 40. Proc Natl Acad Sci U S A. 1980; 77(7):3898-902. PMC: 349734. DOI: 10.1073/pnas.77.7.3898. View

18.

Myers R, Tjian R . Construction and analysis of simian virus 40 origins defective in tumor antigen binding and DNA replication. Proc Natl Acad Sci U S A. 1980; 77(11):6491-5. PMC: 350311. DOI: 10.1073/pnas.77.11.6491. View

19.

Gluzman Y . SV40-transformed simian cells support the replication of early SV40 mutants. Cell. 1981; 23(1):175-82. DOI: 10.1016/0092-8674(81)90282-8. View

20.

Bergsma D, Olive D, Hartzell S, Subramanian K . Territorial limits and functional anatomy of the simian virus 40 replication origin. Proc Natl Acad Sci U S A. 1982; 79(2):381-5. PMC: 345744. DOI: 10.1073/pnas.79.2.381. View